Main Information

Target

Actinin-α1

Host Species

Mouse

Reactivity

Human, Rat, Mouse

Applications

WB, IHC, IF

MW

103kD (Observed)

Conjugate/Modification

Unmodified

Detailed Information

Recommended Dilution Ratio

IHC 1:200-400; IF/ICC1:200-400; WB 1:500-2000

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Specificity

a-actinin Monoclonal Antibody(4A12) detects endogenous levels of ACTN1

Purification

The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

Storage

-15°C to -25°C/1 year(Do not lower than -25°C)

Concentration

1 mg/ml

MW(Observed)

103kD

Modification

Unmodified

Clonality

Monoclonal

Clone Number

4A12

Antigen&Target Information

Immunogen:

Recombinant Protein of a-actinin of ACTN1

Specificity:

a-actinin Monoclonal Antibody(4A12) detects endogenous levels of ACTN1

Gene Name:

ACTN1

Protein Name:

ACTN1

Other Name:

ACTN1

Database Link:

Organism	Gene ID	SwissProt
Human	87;	P12814;
Mouse		Q7TPR4;
Rat		Q9Z1P2;

Background:

Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, cytoskeletal, alpha actinin isoform and maps to the same site as the structurally similar erythroid beta spectrin gene. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008],

Function:

Function:F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein.,similarity:Belongs to the alpha-actinin family.,similarity:Contains 1 actin-binding domain.,similarity:Contains 2 CH (calponin-homology) domains.,similarity:Contains 2 EF-hand domains.,similarity:Contains 4 spectrin repeats.,subcellular location:Colocalizes with MYOZ2 and PPP3CA at the Z-line of heart and skeletal muscle.,subunit:Homodimer; antiparallel. Interacts with DDN, MYOZ2, PDLIM2, TTID and LPP.,

Cellular Localization:

Cytoplasm, cytoskeleton . Cytoplasm, myofibril, sarcomere, Z line . Cell membrane . Cell junction . Cell projection, ruffle . Colocalizes with MYOZ2 and PPP3CA at the Z-line of heart and skeletal muscle. Colocalizes with PSD in membrane ruffles and central reticular structures. .

Tissue Expression:

Colon,Epithelium,Hepatocyte,Neuroblastoma,Placenta,Platelet,Skin,Tumor of tongue,

Research Areas:

>>Focal adhesion ;
>>Adherens junction ;
>>Tight junction ;
>>Leukocyte transendothelial migration ;
>>Regulation of actin cytoskeleton ;
>>Shigellosis ;
>>Amoebiasis ;
>>Viral carcinogenesis ;
>>Systemic lupus erythematosus