Target：HIF-3α

Gene Name：HIF3A

Protein Name：Hypoxia-inducible factor 3-alpha

Human Gene Id：64344

Human Swiss Prot No：Q66K72

Immunogen：The antiserum was produced against synthesized peptide derived from human HIF-3alpha. AA range:305-354

Specificity：HIF-3α Polyclonal Antibody detects endogenous levels of HIF-3α protein.

Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Source：Polyclonal, Rabbit,IgG

Dilution：WB 1:500 - 1:2000. ELISA: 1:20000. Not yet tested in other applications.

Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration：1 mg/ml

Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)

Other Name：Hypoxia-inducible factor 3-alpha;HIF-3-alpha;HIF3-alpha;Basic-helix-loop-helix-PAS protein MOP7;Class E basic helix-loop-helix protein 17;bHLHe17;HIF3-alpha-1;Inhibitory PAS domain protein;IPAS;Member of PAS protein 7;PAS domain-c

Observed Band(KD)：72kD

Background：hypoxia inducible factor 3 alpha subunit(HIF3A) Homo sapiens The protein encoded by this gene is the alpha-3 subunit of one of several alpha/beta-subunit heterodimeric transcription factors that regulate many adaptive responses to low oxygen tension (hypoxia). The alpha-3 subunit lacks the transactivation domain found in factors containing either the alpha-1 or alpha-2 subunits. It is thought that factors containing the alpha-3 subunit are negative regulators of hypoxia-inducible gene expression. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Mar 2011],

Function：function:Involved in adaptive response to hypoxia. Suppresses hypoxia-inducible expression of HIF1A and EPAS1. Binds to core DNA sequence 5'-TACGTG-3' within the hypoxia response element (HRE) of target gene promoters. The complex HIF3A-ARNT activates the transcription of reporter genes driven by HRE. Isoform 4 has a dominant-negative function of inactivating HIF1A-mediated transcription. Isoform 4 attenuates the binding of HIF1A to hypoxia-responsive elements (HRE), thus inhibiting HRE-driven transcription. Hypoxia induces down-regulation of isoform 4, leading to activation of HIF1A in hypoxia. Conversely, upon restoring normoxia, the expression of isoform 4 increases and thereby secure an inhibition of HIF1A activity. Isoform 4 may be a negative regulator of hypoxia-inducible gene expression in the kidney and may be involved in renal tumorigenesis. Functions as an inhibitor of angiogen

Subcellular Location：nucleus,nucleoplasm,mitochondrion,cytosol,nuclear speck,

Expression： Cerebellum,Embryo,Kidney,Ovary,Pancreas,Whole embryo,