Target：Hint1

Gene Name：HINT1

Protein Name：Histidine triad nucleotide-binding protein 1

Human Gene Id：3094

Human Swiss Prot No：P49773

Mouse Gene Id：15254

Mouse Swiss Prot No：P70349

Rat Gene Id：690660

Rat Swiss Prot No：P62959

Immunogen：The antiserum was produced against synthesized peptide derived from human HINT1. AA range:71-120

Specificity：Hint1 Polyclonal Antibody detects endogenous levels of Hint1 protein.

Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Source：Polyclonal, Rabbit,IgG

Dilution：WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.

Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration：1 mg/ml

Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)

Other Name：HINT1;HINT;PKCI1;PRKCNH1;Histidine triad nucleotide-binding protein 1;Adenosine 5'-monophosphoramidase;Protein kinase C inhibitor 1;Protein kinase C-interacting protein 1;PKCI-1

Observed Band(KD)：28kD

Background： This gene encodes a protein that hydrolyzes purine nucleotide phosphoramidates substrates, including AMP-morpholidate, AMP-N-alanine methyl ester, AMP-alpha-acetyl lysine methyl ester, and AMP-NH2. The encoded protein interacts with these substrates via a histidine triad motif. This gene is considered a tumor suppressor gene. In addition, mutations in this gene can cause autosomal recessive neuromyotonia and axonal neuropathy. There are several related pseudogenes on chromosome 7. Several transcript variants have been observed. [provided by RefSeq, Dec 2015],

Function：caution:Was originally thought to be a protein kinase C inhibitor and to bind zinc in solution. Both seem to be incorrect.,domain:The histidine triad, also called HIT motif, forms part of the binding loop for the alpha-phosphate of purine mononucleotide.,function:Hydrolyzes adenosine 5'-monophosphoramidate substrates such as AMP-morpholidate, AMP-N-alanine methyl ester, AMP-alpha-acetyl lysine methyl ester and AMP-NH2.,similarity:Belongs to the HINT family.,similarity:Contains 1 HIT domain.,subcellular location:Interaction with CDK7 leads to a more nuclear localization.,subunit:Homodimer. Interacts with CDK7.,tissue specificity:Widely expressed.,

Subcellular Location：Cytoplasm . Nucleus . Interaction with CDK7 leads to a more nuclear localization. .

Expression：Widely expressed.