Target：SAP 49

Fields：Spliceosome

Gene Name：SF3B4

Protein Name：Splicing factor 3B subunit 4

Human Gene Id：10262

Human Swiss Prot No：Q15427

Mouse Gene Id：107701

Mouse Swiss Prot No：Q8QZY9

Rat Gene Id：295270

Rat Swiss Prot No：Q6AYL5

Immunogen：The antiserum was produced against synthesized peptide derived from human SF3B4. AA range:61-110

Specificity：SAP 49 Polyclonal Antibody detects endogenous levels of SAP 49 protein.

Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Source：Polyclonal, Rabbit,IgG

Dilution：WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:40000.. IF 1:50-200

Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration：1 mg/ml

Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)

Other Name：SF3B4;SAP49;Splicing factor 3B subunit 4;Pre-mRNA-splicing factor SF3b 49 kDa subunit;SF3b50;Spliceosome-associated protein 49;SAP 49

Observed Band(KD)：55kD

Background： This gene encodes one of four subunits of the splicing factor 3B. The protein encoded by this gene cross-links to a region in the pre-mRNA immediately upstream of the branchpoint sequence in pre-mRNA in the prespliceosomal complex A. It also may be involved in the assembly of the B, C and E spliceosomal complexes. In addition to RNA-binding activity, this protein interacts directly and highly specifically with subunit 2 of the splicing factor 3B. This protein contains two N-terminal RNA-recognition motifs (RRMs), consistent with the observation that it binds directly to pre-mRNA. [provided by RefSeq, Jul 2008],

Function：function:Subunit of the splicing factor SF3B required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA. May also be involved in the assembly of the 'E' complex. SF3B4 has been found in complex 'B' and 'C' as well. Belongs also to the minor U12-dependent spliceosome, which is involved in the splicing of rare class of nuclear pre-mRNA intron.,similarity:Belongs to the SF3B4 family.,similarity:Contains 2 RRM (RNA recognition motif) domains.,subunit:Component of splicing factor SF3B which is composed of at least eight subunits; SF3B1/SAP155/SF3B155, SF3B2/SAP145/SF3B145, SF3B3/SAP130/SF3B130, SF3B4/SAP49/SF3B49, SF3B14A, PHF5A/SF3B14B, SF3B10 and SF3B125. SF3B associates with the splicing factor SF3A

Subcellular Location：Nucleus .

Expression： Ovarian carcinoma,Skin,Testis,Thymus,