Target：SAP 155

Fields：Spliceosome

Gene Name：SF3B1

Protein Name：Splicing factor 3B subunit 1

Human Gene Id：23451

Human Swiss Prot No：O75533

Mouse Swiss Prot No：Q99NB9

Immunogen：The antiserum was produced against synthesized peptide derived from human SAP 155. AA range:75-124

Specificity：SAP 155 Polyclonal Antibody detects endogenous levels of SAP 155 protein.

Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Source：Polyclonal, Rabbit,IgG

Dilution：WB 1:500-2000;IHC 1:50-300

Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration：1 mg/ml

Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)

Other Name：SF3B1;SAP155;Splicing factor 3B subunit 1;Pre-mRNA-splicing factor SF3b 155 kDa subunit;SF3b155;Spliceosome-associated protein 155;SAP 155

Observed Band(KD)：145kD

Background： This gene encodes subunit 1 of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3b is also a component of the minor U12-type spliceosome. The carboxy-terminal two-thirds of subunit 1 have 22 non-identical, tandem HEAT repeats that form rod-like, helical structures. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008],

Function：function:Subunit of the splicing factor SF3B required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA. May also be involved in the assembly of the 'E' complex. Belongs also to the minor U12-dependent spliceosome, which is involved in the splicing of rare class of nuclear pre-mRNA intron.,PTM:Phosphorylated. Phosphorylation occurs concomitantly with the splicing catalytic steps. Phosphorylation on Thr-244, Thr-248 and Thr-313 by cyclin-dependent kinases promotes interaction with PPP1R8 during mitosis.,similarity:Belongs to the SF3B1 family.,similarity:Contains 11 HEAT repeats.,subcellular location:During mitosis, transiently dispersed from the nuclear speckles to the cytoplasm.,subunit:Identified

Subcellular Location：Nucleus . Nucleus speckle. During mitosis, transiently dispersed from the nuclear speckles to the cytoplasm.

Expression： Brain,Epithelium,Pooled,Testis,Uterus,