Target：E4BP4

Gene Name：NFIL3

Protein Name：Nuclear factor interleukin-3-regulated protein

Human Gene Id：4783

Human Swiss Prot No：Q16649

Mouse Gene Id：18030

Mouse Swiss Prot No：O08750

Rat Gene Id：114519

Rat Swiss Prot No：Q6IMZ0

Immunogen：The antiserum was produced against synthesized peptide derived from human NFIL3. AA range:61-110

Specificity：E4BP4 Polyclonal Antibody detects endogenous levels of E4BP4 protein.

Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Source：Polyclonal, Rabbit,IgG

Dilution：WB 1:500 - 1:2000. IF 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.

Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration：1 mg/ml

Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)

Other Name：NFIL3;E4BP4;IL3BP1;Nuclear factor interleukin-3-regulated protein;E4 promoter-binding protein 4;Interleukin-3 promoter transcriptional activator;Interleukin-3-binding protein 1;Transcriptional activator NF-IL3A

Observed Band(KD)：51kD

Background： The protein encoded by this gene is a transcriptional regulator that binds as a homodimer to activating transcription factor (ATF) sites in many cellular and viral promoters. The encoded protein represses PER1 and PER2 expression and therefore plays a role in the regulation of circadian rhythm. Three transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Feb 2014],

Function：function:Acts as a transcriptional regulator that recognizes and binds to the sequence 5'-[GA]TTA[CT]GTAA[CT]-3', a sequence present in many cellular and viral promoters. Represses transcription from promoters with activating transcription factor (ATF) sites. Represses promoter activity in osteoblasts (By similarity). Represses transcriptional activity of PER1 (By similarity). Represses transcriptional activity of PER2 via the B-site on the promoter (By similarity). Activates transcription from the interleukin-3 promoter in T-cells. Competes for the same consensus-binding site with PAR DNA-binding factors (DBP, HLF and TEF) (By similarity). Component of the circadian clock that acts as a negative regulator for the circadian expression of PER2 oscillation in the cell-autonomous core clock (By similarity). Protects pro-B cells from programmed cell death.,induction:Up-regulated by PHA or TP

Subcellular Location：Nucleus .

Expression：Expressed in bladder stomach, thyroid, spinal cord, lymph node, trachea, adrenal gland, bone marrow and muscle.