Target：NEIL1

Fields：Base excision repair

Gene Name：NEIL1

Protein Name：Endonuclease 8-like 1

Human Gene Id：79661

Human Swiss Prot No：Q96FI4

Mouse Swiss Prot No：Q8K4Q6

Immunogen：The antiserum was produced against synthesized peptide derived from human NEIL1. AA range:291-340

Specificity：NEIL1 Polyclonal Antibody detects endogenous levels of NEIL1 protein.

Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Source：Polyclonal, Rabbit,IgG

Dilution：WB 1:500 - 1:2000. ELISA: 1:20000. Not yet tested in other applications.

Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration：1 mg/ml

Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)

Other Name：NEIL1;Endonuclease 8-like 1;DNA glycosylase/AP lyase Neil1;DNA-(apurinic or apyrimidinic site) lyase Neil1;Endonuclease VIII-like 1;FPG1;Nei homolog 1;NEH1;Nei-like protein 1

Observed Band(KD)：44kD

Background： This gene is a member of the Nei endonuclease VIII-like gene family which encodes DNA glycosylases. The encoded enzyme participates in the DNA repair pathway by initiating base excision repair by removing damaged bases, primarily oxidized pyrimidines. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2012],

Function：catalytic activity:Removes damaged bases from DNA, leaving an abasic site.,catalytic activity:The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.,function:Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Acts as DNA glycosylase that recognizes and removes damaged bases. Has a preference for oxidized pyrimidines, such as thymine glycol, formamidopyrimidine (Fapy) and 5-hydroxyuracil. Has marginal activity towards 8-oxoguanine. Has AP (apurinic/apyrimidinic) lyase activity and introduces nicks in the DNA strand. Cleaves the DNA backbone by beta-delta elimination to generate a single-strand break at the site of the removed base with both 3'- and 5'-phosphates. Has DNA glycosylase/lyase activity towards mismatched uracil a

Subcellular Location：Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Nucleus . Chromosome . During mitosis, associates with centrosomes and condensed chromatin.

Expression：Ubiquitous.