Target:PSK-H1
Gene Name:PSKH1
Protein Name:Serine/threonine-protein kinase H1
Human Gene Id:5681
Human Swiss Prot No:P11801
Mouse Gene Id:244631
Mouse Swiss Prot No:Q91YA2
Immunogen:The antiserum was produced against synthesized peptide derived from human KPSH1. AA range:261-310
Specificity:PSK-H1 Polyclonal Antibody detects endogenous levels of PSK-H1 protein.
Formulation:Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source:Polyclonal, Rabbit,IgG
Dilution:WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:20000.. IF 1:50-200
Purification:The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration:1 mg/ml
Storage Stability:-15°C to -25°C/1 year(Do not lower than -25°C)
Other Name:PSKH1;Serine/threonine-protein kinase H1;Protein serine kinase H1;PSK-H1
Observed Band(KD):48kD
Background:catalytic activity:ATP + a protein = ADP + a phosphoprotein.,enzyme regulation:Activity depends on Ca(2+) concentration.,function:May be a SFC-associated serine kinase (splicing factor compartment-associated serine kinase) with a role in intranuclear SR protein (non-snRNP splicing factors containing a serine/arginine-rich domain) trafficking and pre-mRNA processing.,PTM:Autophosphorylated on serine residues.,PTM:Myristoylated. Required for membrane association. Prerequisite for palmitoylation to occur.,PTM:Palmitoylated.,similarity:Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.,similarity:Contains 1 protein kinase domain.,subcellular location:Localized in the Brefeldin A-sensitive Golgi compartment, at centrosomes, in the nucleus with a somewhat speckle-like presence, membrane-associated to the endoplasmic reticulum (ER) and the plasma membrane (PM), and more diffusely in the cytoplasm. Found to concentrate in splicing factor compartments (SFCs) within the nucleus of interphase cells. The acylation-negative form may be only cytoplasmic and nuclear. Acylation seems to allow the sequestering to the intracellular membranes. Myristoylation may mediate targeting to the intracellular non-Golgi membranes and palmitoylation may mediate the targeting to the Golgi membranes. Dual acylation is required to stabilize the interaction with Golgi membranes.,subunit:Homodimer.,tissue specificity:Expressed in all tissues and cell lines tested with the highest level of abundance in testis.,
Function:catalytic activity:ATP + a protein = ADP + a phosphoprotein.,enzyme regulation:Activity depends on Ca(2+) concentration.,function:May be a SFC-associated serine kinase (splicing factor compartment-associated serine kinase) with a role in intranuclear SR protein (non-snRNP splicing factors containing a serine/arginine-rich domain) trafficking and pre-mRNA processing.,PTM:Autophosphorylated on serine residues.,PTM:Myristoylated. Required for membrane association. Prerequisite for palmitoylation to occur.,PTM:Palmitoylated.,similarity:Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.,similarity:Contains 1 protein kinase domain.,subcellular location:Localized in the Brefeldin A-sensitive Golgi compartment, at centrosomes, in the nucleus with a somewhat speckle-like presence, membrane-associated to the endoplasmic reticulum (ER) and the plasma membrane (PM), and m
Subcellular Location:Golgi apparatus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Nucleus speckle. Endoplasmic reticulum membrane; Lipid-anchor. Cell membrane; Lipid-anchor. Cytoplasm. Localized in the brefeldin A-sensitive Golgi compartment, at centrosomes, in the nucleus with a somewhat speckle-like presence, membrane-associated to the endoplasmic reticulum (ER) and the plasma membrane (PM), and more diffusely in the cytoplasm. Found to concentrate in splicing factor compartments (SFCs) within the nucleus of interphase cells. The acylation-negative form may be only cytoplasmic and nuclear. Acylation seems to allow the sequestering to the intracellular membranes. Myristoylation may mediate targeting to the intracellular non-Golgi membranes and palmitoylation may mediate the targeting t
Expression:Expressed in all tissues and cell lines tested with the highest level of abundance in testis.