Target：TRIM3

Gene Name：TRIM3

Protein Name：Tripartite motif-containing protein 3

Human Gene Id：10612

Human Swiss Prot No：O75382

Mouse Gene Id：55992

Mouse Swiss Prot No：Q9R1R2

Rat Gene Id：83616

Rat Swiss Prot No：O70277

Immunogen：The antiserum was produced against synthesized peptide derived from human TRIM3. AA range:1-50

Specificity：TRIM3 Polyclonal Antibody detects endogenous levels of TRIM3 protein.

Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Source：Polyclonal, Rabbit,IgG

Dilution：WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:40000. Not yet tested in other applications.

Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration：1 mg/ml

Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)

Other Name：TRIM3;BERP;RNF22;RNF97;Tripartite motif-containing protein 3;Brain-expressed RING finger protein;RING finger protein 22;RING finger protein 97

Observed Band(KD)：81kD

Background： The protein encoded by this gene is a member of the tripartite motif (TRIM) family, also called the 'RING-B-box-coiled-coil' (RBCC) subgroup of RING finger proteins. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This protein localizes to cytoplasmic filaments. It is similar to a rat protein which is a specific partner for the tail domain of myosin V, a class of myosins which are involved in the targeted transport of organelles. The rat protein can also interact with alpha-actinin-4. Thus it is suggested that this human protein may play a role in myosin V-mediated cargo transport. Alternatively spliced transcript variants encoding the same isoform have been identified. [provided by RefSeq, Jul 2008],

Function：domain:The interaction with myosin V is dependent upon its NHL repeats, which form a beta-propeller (NHL) domain containing six blades.,similarity:Belongs to the TRIM/RBCC family.,similarity:Contains 1 B box-type zinc finger.,similarity:Contains 1 filamin repeat.,similarity:Contains 1 RING-type zinc finger.,similarity:Contains 6 NHL repeats.,subunit:Associates with myosin V and alpha-actinin-4 (ACTN4).,tissue specificity:Expressed in brain, heart, uterus and testis.,

Subcellular Location：Cytoplasm . Early endosome . Golgi apparatus, trans-Golgi network . Cell projection, dendrite .

Expression：Expressed in brain, heart, uterus and testis.