Target：TRAP230

Fields：Thyroid hormone signaling pathway

Gene Name：MED12

Protein Name：Mediator of RNA polymerase II transcription subunit 12

Human Gene Id：9968

Human Swiss Prot No：Q93074

Mouse Swiss Prot No：A2AGH6

Immunogen：The antiserum was produced against synthesized peptide derived from human MED12. AA range:611-660

Specificity：TRAP230 Polyclonal Antibody detects endogenous levels of TRAP230 protein.

Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Source：Polyclonal, Rabbit,IgG

Dilution：WB 1:500-2000 IHC 1:100 - 1:300. ELISA: 1:5000.. IF 1:50-200

Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration：1 mg/ml

Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)

Other Name：MED12;ARC240;CAGH45;HOPA;KIAA0192;TNRC11;TRAP230;Mediator of RNA polymerase II transcription subunit 12;Activator-recruited cofactor 240 kDa component;ARC240;CAG repeat protein 45;Mediator complex subunit 12;OPA-containing prote

Molecular Weight(Da)：247kD

Background： The initiation of transcription is controlled in part by a large protein assembly known as the preinitiation complex. A component of this preinitiation complex is a 1.2 MDa protein aggregate called Mediator. This Mediator component binds with a CDK8 subcomplex which contains the protein encoded by this gene, mediator complex subunit 12 (MED12), along with MED13, CDK8 kinase, and cyclin C. The CDK8 subcomplex modulates Mediator-polymerase II interactions and thereby regulates transcription initiation and reinitation rates. The MED12 protein is essential for activating CDK8 kinase. Defects in this gene cause X-linked Opitz-Kaveggia syndrome, also known as FG syndrome, and Lujan-Fryns syndrome. [provided by RefSeq, Aug 2009],

Function：disease:Defects in MED12 are the cause of Lujan-Fryns syndrome [MIM:309520]; also known as X-linked mental retardation with marfanoid habitus. Clinically, Lujan-Fryns syndrome can be distinguished from Opitz-Kaveggia syndrome by tall stature, hypernasal voice, hyperextensible digits and high nasal root.,disease:Defects in MED12 are the cause of Opitz-Kaveggia syndrome (OKS) [MIM:305450]; also known as FG syndrome type 1 (FGS1) or FG syndrome (FGS). OKS is an X-linked disorder characterized by mental retardation, relative macrocephaly, hypotonia and constipation.,function:Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters b

Subcellular Location：Nucleus .

Expression：Ubiquitous.