Main Information

Target

Smad3

Host Species

Mouse

Reactivity

Human, Mouse, Rat

Applications

IHC, IF

MW

52kD (Observed)

Conjugate/Modification

Phospho

Detailed Information

Recommended Dilution Ratio

IHC 1:50-300; IF 1:50-200

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Specificity

The antibody detects endogenous Phospho-Smad3(S425) protein

Purification

The antibody was affinity-purified from mouse antiserum by affinity-chromatography using epitope-specific immunogen.

Storage

-15°C to -25°C/1 year(Do not lower than -25°C)

Concentration

1 mg/ml

MW(Observed)

52kD

Modification

Phospho

Clonality

Monoclonal

Clone Number

1D9

Antigen&Target Information

Immunogen:

Synthetic Peptide of Phospho-Smad3(S425)

Specificity:

The antibody detects endogenous Phospho-Smad3(S425) protein

Gene Name:

SMAD3 MADH3

Protein Name:

Mothers against decapentaplegic homolog 3 (MAD homolog 3) (Mad3) (Mothers against DPP homolog 3) (hMAD-3) (JV15-2) (SMAD family member 3) (SMAD 3) (Smad3) (hSMAD3)

Other Name:

Mothers against decapentaplegic homolog 3 ;
MAD homolog 3 ;
Mad3 ;
Mothers against DPP homolog 3 ;
hMAD-3 ;
JV15-2 ;
SMAD family member 3 ;
SMAD 3 ;
Smad3 ;
hSMAD3 ;

Database Link:

Organism	Gene ID	SwissProt
Human	4088;	P84022;
Mouse		Q8BUN5;
Rat		P84025;

Background:

The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions as a transcriptional modulator activated by transforming growth factor-beta and is thought to play a role in the regulation of carcinogenesis. [provided by RefSeq, Apr 2009],

Function:

Disease:Defects in SMAD3 may be a cause of colorectal cancer (CRC) [MIM:114500].,Domain:The MH2 domain is sufficient to carry protein nuclear export.,Function:Transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinase. SMAD3 is a receptor-regulated SMAD (R-SMAD).,PTM:Phosphorylated on serine by TGF-beta and activin type 1 receptor kinases.,similarity:Belongs to the dwarfin/SMAD family.,similarity:Contains 1 MH1 (MAD homology 1) domain.,similarity:Contains 1 MH2 (MAD homology 2) domain.,subcellular location:In the cytoplasm in the absence of ligand. Migration to the nucleus when complexed with Smad4.,subunit:Interacts with HGS. Interacts with NEDD4L in response to TGF-beta. Interacts with TTRAP (By similarity). Interacts with SARA (SMAD anchor for receptor activation); form trimers with another SMAD3 and the co-SMAD SMAD4. Interacts with JUN/FOS, vitamin D receptor, homeobox protein TGIF and TGIF2, PEBP2-alpha C subunit, CREB-binding protein (CBP), p300, SKI, SNON, ATF2, SMURF2, AIP1, DACH1 and TGFB1I1. Part of a complex consisting of AIP1, ACVR2A, ACVR1B and SMAD3. Found in a complex with SMAD2 and TRIM33 upon addition of TGF-beta. Interacts with SMAD2 and TRIM33. Found in a complex with SMAD3, Ran and XPO4. Interacts with XPO4. Interacts with LBXCOR1 and CORL2.,

Cellular Localization:

Cytoplasm . Nucleus . Cytoplasmic and nuclear in the absence of TGF-beta. On TGF-beta stimulation, migrates to the nucleus when complexed with SMAD4 (PubMed:15799969, PubMed:21145499). Through the action of the phosphatase PPM1A, released from the SMAD2/SMAD4 complex, and exported out of the nucleus by interaction with RANBP1 (PubMed:16751101, PubMed:19289081). Co-localizes with LEMD3 at the nucleus inner membrane (PubMed:15601644). MAPK-mediated phosphorylation appears to have no effect on nuclear import (PubMed:19218245). PDPK1 prevents its nuclear translocation in response to TGF-beta (PubMed:17327236). Localized mainly to the nucleus in the early stages of embryo development with expression becoming evident in the cytoplasm of the inner cell mass at the blastocyst stage (By similarity). .

Tissue Expression:

Brain,Colon carcinoma,Esophagus tumor,Pancreas,Placenta,Spleen,Umbilical cord blood

Research Areas:

>>FoxO signaling pathway ;
>>Cell cycle ;
>>Endocytosis ;
>>Cellular senescence ;
>>Wnt signaling pathway ;
>>TGF-beta signaling pathway ;
>>Apelin signaling pathway ;
>>Hippo signaling pathway ;
>>Adherens junction ;
>>Signaling pathways regulating pluripotency of stem cells ;
>>Th17 cell differentiation ;
>>AGE-RAGE signaling pathway in diabetic complications ;
>>Hepatitis B ;
>>Human T-cell leukemia virus 1 infection ;
>>Pathways in cancer ;
>>Colorectal cancer ;
>>Pancreatic cancer ;
>>Chronic myeloid leukemia ;
>>Hepatocellular carcinoma ;
>>Gastric cancer ;
>>Inflammatory bowel disease ;
>>Diabetic cardiomyopathy