Main Information

Target

MLKL

Host Species

Mouse

Reactivity

Human

Applications

IF, IHC

MW

54kD (Observed)

Conjugate/Modification

Phospho

Detailed Information

Recommended Dilution Ratio

IF 1:50-200; IHC 1:100-200

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Specificity

phospho-MLKL (S358) protein detects endogenous levels of MLKL

Purification

The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

Storage

-15°C to -25°C/1 year(Do not lower than -25°C)

Concentration

1 mg/ml

MW(Observed)

54kD

Modification

Phospho

Clonality

Monoclonal

Clone Number

6F8

Antigen&Target Information

Immunogen:

Synthetic Peptide of phospho-MLKL (S358)

Specificity:

phospho-MLKL (S358) protein detects endogenous levels of MLKL

Gene Name:

MLKL

Protein Name:

MLKL

Other Name:

MLKL

Database Link:

Organism	Gene ID	SwissProt
Human	197259;	Q8NB16;
Mouse		Q9D2Y4;

Background:

This gene belongs to the protein kinase superfamily. The encoded protein contains a protein kinase-like domain; however, is thought to be inactive because it lacks several residues required for activity. This protein plays a critical role in tumor necrosis factor (TNF)-induced necroptosis, a programmed cell death process, via interaction with receptor-interacting protein 3 (RIP3), which is a key signaling molecule in necroptosis pathway. Inhibitor studies and knockdown of this gene inhibited TNF-induced necrosis. High levels of this protein and RIP3 are associated with inflammatory bowel disease in children. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Sep 2015],

Function:

Domain:The protein kinase domain is predicted to be catalytically inactive.,similarity:Belongs to the protein kinase superfamily.,similarity:Contains 1 protein kinase domain.,

Cellular Localization:

Cytoplasm . Cell membrane . Nucleus . Localizes to the cytoplasm and translocates to the plasma membrane on necroptosis induction (PubMed:24316671). Localizes to the nucleus in response to orthomyxoviruses infection (By similarity). .

Tissue Expression:

Chondrocyte,Leukocyte,Lymph node,

Research Areas:

>>Necroptosis ;
>>TNF signaling pathway ;
>>Salmonella infection