Main Information

Target

Rabbit IgG (Fc specific_Heavy Chain)

Host Species

Mouse

Reactivity

Rabbit

Applications

WB

MW

55kD (Observed)

Conjugate/Modification

Unmodified

Detailed Information

Recommended Dilution Ratio

WB 1:2000-5000

Formulation

PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.

Specificity

The antibody reacts with the Fc portion of rabbit IgG heavy chain but not with the Fab portion of rabbit immunoglobulins. The antibody may cross-react with immunoglobulins from other species.

Purification

The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

Storage

-15°C to -25°C/1 year(Do not lower than -25°C)

MW(Observed)

55kD

Modification

Unmodified

Clonality

Monoclonal

Clone Number

1E1

Antigen&Target Information

Immunogen:

Recombinant Protein of Rabbit IgG (Fc specific, Heavy Chain)

Specificity:

The antibody reacts with the Fc portion of rabbit IgG heavy chain but not with the Fab portion of rabbit immunoglobulins. The antibody may cross-react with immunoglobulins from other species.

Background:

Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa.The Fc specific, Heavy Chain antibody reacts with the Fc portion of IgG heavy chain but not with the Fab portion of IgG.