Main Information

Target

Rabbit IgG (H+L)

Host Species

Mouse

Reactivity

Rabbit

Applications

WB

MW

25,55kD (Observed)

Conjugate/Modification

Unmodified

Detailed Information

Recommended Dilution Ratio

WB 1:2000-5000

Formulation

PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.

Specificity

The antibody reacts with whole molecule rabbit IgG. The antibody may cross-react with immunoglobulins from other species.

Purification

The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

Storage

-15°C to -25°C/1 year(Do not lower than -25°C)

MW(Observed)

25,55kD

Modification

Unmodified

Clonality

Monoclonal

Antigen&Target Information

Immunogen:

Recombinant Protein of Rabbit IgG (H+L)

Specificity:

The antibody reacts with whole molecule rabbit IgG. The antibody may cross-react with immunoglobulins from other species.

Background:

Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective.