Main Information

Target

GST-Tag

Host Species

Mouse

Reactivity

Species independent

Applications

WB, ELISA, IP

Conjugate/Modification

Unmodified

Detailed Information

Recommended Dilution Ratio

WB 1:5000; IP 1:200-500; ELISA 1:5000-20000

Formulation

PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.

Specificity

The antibody detects C-terminal, internal, and N-terminal GST fusion proteins.

Purification

The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

Storage

-15°C to -25°C/1 year(Do not lower than -25°C)

Modification

Unmodified

Clonality

Monoclonal

Clone Number

1B10

Antigen&Target Information

Immunogen:

Recombinant Protein of GST-Tag

Specificity:

The antibody detects C-terminal, internal, and N-terminal GST fusion proteins.

Background:

The glutathione S-transferase (GST, previously known as ligandins) family of enzymes are composed of many cytosolic, mitochondrial, and microsomal proteins. GSTs are present in eukaryotes and in prokaryotes, where they catalyze a variety of reactions and accept endogenous and xenobiotic substrates. GST tag antibody can be helpful in detecting the fusion protein during purification as well as the cleavage of GST from the protein of interest. GST tag antibody has wide applications that could include your research on GST proteins or GST fusion recombinant proteins.