Main Information

Target

His-Tag

Host Species

Mouse

Reactivity

Species independent

Applications

WB, IP, IF

Conjugate/Modification

Unmodified

Detailed Information

Recommended Dilution Ratio

WB 1:3000; IP 1:200; IF 1:1000

Formulation

PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.

Specificity

The antibody detects C-terminal, internal, and N-terminal His-tag fusion proteins.

Purification

The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

Storage

-15°C to -25°C/1 year(Do not lower than -25°C)

Modification

Unmodified

Clonality

Monoclonal

Clone Number

4E6

Antigen&Target Information

Immunogen:

Synthetic peptide:HHHHHH conjugated to KLH.

Specificity:

The antibody detects C-terminal, internal, and N-terminal His-tag fusion proteins.

Gene Name:

His-Tag

Protein Name:

His Tag

Background:

A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein. It is also known as hexa histidine-tag, 6xHis-tag, and by the trademarked name His-tag. Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokaryotic expression systems.