产品简介:
pBAD/His A is a pBR322-derived expression vector designed for regulated, dose-dependent recombinant protein expression and purification in E. coli. Optimum levels of soluble, recombinant protein are possible using the araBAD promoter (PBAD) from E. coli. The regulatory protein, AraC, is provided on the pBAD/His A vector allowing regulation of PBAD. In the presence of L-arabinose, expression from PBAD is turned on while the absence of L-arabinose produces very low levels of transcription from PBAD.
产品参数:
质粒类型:大肠杆菌表达载体
出品公司:Invitrogen
表达水平:高拷贝克隆方法:多克隆位点,限制性内切酶
启动子:araBad
载体大小:4102 bp5' 测序引物及序列:pBAD-F:ATGCCATAGCATTTTTATCC
3' 测序引物及序列:pBAD-R:CACTTCTGAGTTCGGCATGG
载体标签:N-His, N-EK,N-xPress Epitope
载体抗性:Ampicillin (氨苄青霉素)
诱导方式:阿拉伯糖
注意事项:
1、为了您的安全和健康,请穿实验服并戴一次性手套操作。本产品仅限于专业人员的科学研究用,不得用于临床诊断或治疗等其他用途。
2、使用甘油菌可不完全融解,在菌液表面蘸取少量涂布固体琼脂平板即可。完全融解后使用,菌体会逐渐沉淀至管底,请混匀后使用。随着冻融次数的增加,菌株的活力会逐渐下降。