产品简介：
The Glutathione S-transferase (GST) Gene Fusion System from GE Healthcare is a versatile system for the expression, purification, and detection of GST-tagged proteins produced in E. coli. GST occurs naturally as an Mr 26 000 protein, which can be expressed in E. coli with full enzymatic activity. The pGEX plasmid vectors are designed for inducible, high-level intracellular expression of genes or gene fragments as fusions with S. japonicum GST. Recombinant proteins are easily purified from, for example E. coli cell lysates by affinity chromatography using Glutathione Sepharose media, either with prepacked columns or by batch purification. Cleavage of the target protein from GST is achieved using a site-specific protease, which possesses a recognition sequence located immediately upstream from the multiple cloning site on the pGEX vectors.

 

产品参数：

质粒类型：大肠杆菌表达载体

出品公司：GE Healthcare
表达水平：高拷贝

克隆方法：多克隆位点，限制性内切酶

启动子：Tac

载体大小：4969 bp

5' 测序引物及序列：pGEX5': GGGCTGGCAAGCCACGTTTGGTG
3' 测序引物及序列：pGEX3': CCGGGAGCTGCATGTGTCAGAGG
载体标签：N-GST
载体抗性：Ampicillin (氨苄青霉素)
备注: 可用GST亲和柱纯化重组蛋白

 

注意事项：
1、为了您的安全和健康，请穿实验服并戴一次性手套操作。本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗等其他用途。
2、使用甘油菌可不完全融解，在菌液表面蘸取少量涂布固体琼脂平板即可。完全融解后使用，菌体会逐渐沉淀至管底，请混匀后使用。随着冻融次数的增加，菌株的活力会逐渐下降。