pGL4.17[luc2/Neo] 载体

质粒类型: 启动子报告载体

载体大小: 5599 bp (查看载体序列)

载体抗性: Ampicillin (氨苄青霉素)

筛选标记: Neomycin (新霉素)

出品公司： Promega

备注: Promoterless vector for measuring the activity of promoter and enhancer sequences with a luciferase assay. 

载体描述

The pGL4.17[luc2/Neo] Vector(a–e)encodes the luciferase reporter gene luc2 (Photinus pyralis) and is designed for high expression and reduced anomalous transcription. This vector also contains a mammalian selectable marker for neomycin resistance in which the number of transcription factor binding sites has been reduced and mammalian codon usage optimized. This vector is also engineered with fewer consensus regulatory sequences for reduced background and a decreased risk of anomolous transcription and has a synthetic reporter gene, which has been codon optimized for mammalian expression.

The pGL4.17[luc2/Neo] Vector is a basic vector with no promoter. However, it contains a multiple cloning region to allow cloning of a promoter of choice.