pGL4.26[luc2/minP/Hygro] 载体
质粒类型: 启动子报告载体
克隆方法: 多克隆位点,限制性内切酶
载体大小: 5882 bp (查看载体序列)
载体抗性: Ampicillin (氨苄青霉素)
筛选标记: Hygromycin (潮霉素)
出品公司:Promega
备注: Vector with a minimal promoter for measuring the activity of transcriptional response elements using a luciferase assay.
载体描述
The pGL4.26[luc2/minP/Hygro] Vector(a–e) encodes the luciferase reporter gene luc2 (Photinus pyralis) and is designed for high expression and reduced anomalous transcription. The vector contains a multiple cloning region for insertion of a response element of interest upstream of a minimal promoter and the luc2 gene. luc2 is a synthetically-derived luciferase sequence with humanized codon optimization. The vector backbone contains an ampicillin resistance gene to allow selection in E. coli and a mammalian selectable marker for hygromycin resistance.