Target：TERF1
Gene Name：TERF1
Protein Name：Telomeric repeat-binding factor 1
Human Gene Id：7013
Human Swiss Prot No：P54274
Mouse Gene Id：21749
Mouse Swiss Prot No：P70371
Immunogen：The antiserum was produced against synthesized peptide derived from human Telomeric Repeat Binding Factor 1 around the phosphorylation site of Ser219. AA range:185-234
Specificity：Phospho-TRF1 (S219) Polyclonal Antibody detects endogenous levels of TRF1 protein only when phosphorylated at S219.
Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source：Polyclonal, Rabbit,IgG
Dilution：WB 1:500 - 1:2000. ELISA: 1:5000. Not yet tested in other applications.
Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration：1 mg/ml
Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)
Other Name：TERF1;PIN2;TRBF1;TRF;TRF1;Telomeric repeat-binding factor 1;NIMA-interacting protein 2;TTAGGG repeat-binding factor 1;Telomeric protein Pin2/TRF1
Observed Band(KD)：55kD
Background： This gene encodes a telomere specific protein which is a component of the telomere nucleoprotein complex. This protein is present at telomeres throughout the cell cycle and functions as an inhibitor of telomerase, acting in cis to limit the elongation of individual chromosome ends. The protein structure contains a C-terminal Myb motif, a dimerization domain near its N-terminus and an acidic N-terminus. Two transcripts of this gene are alternatively spliced products. [provided by RefSeq, Jul 2008],
Function：domain:The acidic N-terminal domain binds to the ankyrin repeats of TNKS1 and TNKS2. The C-terminal domain binds microtubules.,function:Binds the telomeric double-stranded TTAGGG repeat and negatively regulates telomere length. Involved in the regulation of the mitotic spindle. Component of the shelterin complex (telosome) that is involved in the regulation of telomere length and protection. Shelterin associates with arrays of double-stranded TTAGGG repeats added by telomerase and protects chromosome ends; without its protective activity, telomeres are no longer hidden from the DNA damage surveillance and chromosome ends are inappropriately processed by DNA repair pathways.,induction:Pin2 expression is tightly regulated during the cell cycle; levels are low in G1 and S phase and increase during G2 phase and mitosis.,PTM:ADP-ribosylation by TNKS1 or TNKS2 diminishes its ability to bind to
Subcellular Location：Nucleus. Cytoplasm, cytoskeleton, spindle. Chromosome, telomere. Colocalizes with telomeric DNA in interphase and prophase cells. Telomeric localization decreases in metaphase, anaphase and telophase. Associates with the mitotic spindle.
Expression：Highly expressed and ubiquitous. Isoform Pin2 predominates.
