Target：TRAP220
Fields：Endocrine resistance;Thyroid hormone signaling pathway
Gene Name：MED1
Protein Name：Mediator of RNA polymerase II transcription subunit 1
Human Gene Id：5469
Human Swiss Prot No：Q15648
Mouse Gene Id：19014
Mouse Swiss Prot No：Q925J9
Immunogen：The antiserum was produced against synthesized peptide derived from human PPAR-BP around the phosphorylation site of Thr1457. AA range:1423-1472
Specificity：Phospho-TRAP220 (T1457) Polyclonal Antibody detects endogenous levels of TRAP220 protein only when phosphorylated at T1457.
Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source：Polyclonal, Rabbit,IgG
Dilution：WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:5000. Not yet tested in other applications.
Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration：1 mg/ml
Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)
Other Name：MED1;ARC205;CRSP1;CRSP200;DRIP205;DRIP230;PBP;PPARBP;PPARGBP;RB18A;TRAP220;TRIP2;Mediator of RNA polymerase II transcription subunit 1;Activator-recruited cofactor 205 kDa component;ARC205;Mediator complex subunit 1;Peroxiso
Observed Band(KD)：168kD
Background： The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. It also regulates p53-dependent apoptosis and it is essential for adipogenesis. This protein is known to have the ability to self-oligomerize. [provided by RefSeq, Jul 2008],
Function：function:Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.,PTM:Phosphorylated by MAPK1 or MAPK3 during G2/M phase which may enhance protein stability and promote entry into the nucleolus. Phosphorylated upon DNA damage, probably by ATM or ATR.,sequence caution:Contaminating sequence. Potential poly-A sequence.,similarity:Belongs to the Mediator complex subunit 1 family.,subcellular location:A subset of the protein may enter the nucleol
Subcellular Location：Nucleus . A subset of the protein may enter the nucleolus subsequent to phosphorylation by MAPK1 or MAPK3.
Expression：Ubiquitously expressed.
