Target：HNK-1ST

Fields：Mannose type O-glycan biosynthesis;Metabolic pathways

Gene Name：CHST10

Protein Name：Carbohydrate sulfotransferase 10

Human Gene Id：9486

Human Swiss Prot No：O43529

Mouse Gene Id：98388

Mouse Swiss Prot No：Q6PGK7

Rat Gene Id：140568

Rat Swiss Prot No：O54702

Immunogen：The antiserum was produced against synthesized peptide derived from human CHST10. AA range:191-240

Specificity：HNK-1ST Polyclonal Antibody detects endogenous levels of HNK-1ST protein.

Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Source：Polyclonal, Rabbit,IgG

Dilution：WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:40000.. IF 1:50-200

Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration：1 mg/ml

Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)

Other Name：CHST10;Carbohydrate sulfotransferase 10;HNK-1 sulfotransferase;HNK-1ST;HNK1ST;HuHNK-1ST

Observed Band(KD)：48kD

Background： This protein encoded by this gene transfers sulfate to the C-3 hydroxyl of terminal glucuronic acid of protein- and lipid-linked oligosaccharides. This protein was first identified as a sulfotransferase that acts on the human natural killer-1 (HNK-1) glycan; HNK-1 is a carbohydrate involved in neurodevelopment and synaptic plasticity.[provided by RefSeq, Feb 2011],

Function：function:Catalyzes the transfer of sulfate to position 3 of terminal glucuronic acid of both protein- and lipid-linked oligosaccharides. Participates in biosynthesis of HNK-1 carbohydrate structure, a sulfated glucuronyl-lactosaminyl residue carried by many neural recognition molecules, which is involved in cell interactions during ontogenetic development and in synaptic plasticity in the adult. May be indirectly involved in synapse plasticity of the hippocampus, via its role in HNK-1 biosynthesis.,similarity:Belongs to the sulfotransferase 2 family.,tissue specificity:In fetal tissues, it is predominantly expressed in brain, and weakly expressed in lung, kidney and liver. In adult, it is highly expressed in brain, testis, ovary, expressed at intermediate level in heart, pancreas, skeletal muscle, spleen and thymus, and weakly expressed in other tissues. In brain, it is expressed at high

Subcellular Location：Golgi apparatus membrane ; Single-pass type II membrane protein .

Expression：In fetal tissues, it is predominantly expressed in brain, and weakly expressed in lung, kidney and liver. In adult, it is highly expressed in brain, testis, ovary, expressed at intermediate level in heart, pancreas, skeletal muscle, spleen and thymus, and weakly expressed in other tissues. In brain, it is expressed at higher level in the frontal lobe.