Target：Smad6

Fields：TGF-beta signaling pathway

Gene Name：SMAD6

Protein Name：Mothers against decapentaplegic homolog 6

Human Gene Id：4091

Human Swiss Prot No：O43541

Mouse Gene Id：17130

Mouse Swiss Prot No：O35182

Immunogen：The antiserum was produced against synthesized peptide derived from human Smad6. AA range:323-372

Specificity：Smad6 Polyclonal Antibody detects endogenous levels of Smad6 protein.

Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Source：Polyclonal, Rabbit,IgG

Dilution：WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:40000.. IF 1:50-200

Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration：1 mg/ml

Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)

Other Name：SMAD6;MADH6;Mothers against decapentaplegic homolog 6;MAD homolog 6;Mothers against DPP homolog 6;SMAD family member 6;SMAD 6;Smad6;hSMAD6

Observed Band(KD)：62kD

Background： The protein encoded by this gene belongs to the SMAD family of proteins, which are related to Drosophila 'mothers against decapentaplegic' (Mad) and C. elegans Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions in the negative regulation of BMP and TGF-beta/activin-signalling. Multiple transcript variants have been found for this gene.[provided by RefSeq, Sep 2014],

Function：function:Antagonist of signaling by TGF-beta (transforming growth factor) type 1 receptor superfamily members; has been shown to inhibit selectively BMP (bone morphogenetic proteins) signaling by competing with the co-SMAD SMAD4 for receptor-activated SMAD1. SMAD6 is an inhibitory SMAD (I-SMAD) or antagonistic SMAD. Binds to regulatory elements in target promoter regions.,PTM:Phosphorylated by BMP type 1 receptor kinase and by PRKX.,similarity:Belongs to the dwarfin/SMAD family.,similarity:Contains 1 MH1 (MAD homology 1) domain.,similarity:Contains 1 MH2 (MAD homology 2) domain.,subunit:Interacts with NEDD4L (By similarity). Interacts with RNF111 and AXIN1. Interacts with TGF-beta type I receptor superfamily members, SMAD1, HOXC8 and HOXC9. Interacts with STAMBP and PRKX.,tissue specificity:Ubiquitous in various organs, with higher levels in lung. Isoform B is up-regulated in diseased he

Subcellular Location：Nucleus .

Expression：Ubiquitous in various organs, with higher levels in lung. Isoform B is up-regulated in diseased heart tissue.