Target：ACOT8

Fields：Primary bile acid biosynthesis;Metabolic pathways;Peroxisome

Gene Name：ACOT8

Protein Name：Acyl-coenzyme A thioesterase 8

Human Gene Id：10005

Human Swiss Prot No：O14734

Mouse Swiss Prot No：P58137

Immunogen：The antiserum was produced against synthesized peptide derived from human ACOT8. AA range:131-180

Specificity：ACOT8 Polyclonal Antibody detects endogenous levels of ACOT8 protein.

Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Source：Polyclonal, Rabbit,IgG

Dilution：WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:40000. Not yet tested in other applications.

Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration：1 mg/ml

Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)

Other Name：ACOT8;ACTEIII;PTE1;PTE2;Acyl-coenzyme A thioesterase 8;Acyl-CoA thioesterase 8;Choloyl-coenzyme A thioesterase;HIV-Nef-associated acyl-CoA thioesterase;PTE-2;Peroxisomal acyl-coenzyme A thioester hydrolase 1;PTE-1;Peroxisomal lon

Observed Band(KD)：36kD

Background： The protein encoded by this gene is a peroxisomal thioesterase that appears to be involved more in the oxidation of fatty acids rather than in their formation. The encoded protein can bind to the human immunodeficiency virus-1 protein Nef, and mediate Nef-induced down-regulation of CD4 in T-cells. [provided by RefSeq, Oct 2010],

Function：catalytic activity:Choloyl-CoA + H(2)O = cholate + CoA.,function:Acyl-CoA thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH. May mediate Nef-induced down-regulation of CD4. Major thioesterase in peroxisomes. Competes with BAAT (Bile acid CoA: amino acid N-acyltransferase) for bile acid-CoA substrate (such as chenodeoxycholoyl-CoA). Shows a preference for medium-length fatty acyl-CoAs (By similarity). May be involved in the metabolic regulation of peroxisome proliferation.,induction:Regulated by peroxisome proliferator (such as Clofibrate), via the peroxisome proliferator-activated receptors (PPARs).,similarity:Belongs to the C/M/P thioester hydrolase family.,subunit:Interacts with HIV-1 Nef.,tissue specificity:Detect

Subcellular Location：Peroxisome matrix . Predominantly localized in the peroxisome but a localization to the cytosol cannot be excluded. .

Expression：Detected in a T-cell line (at protein level). Ubiquitous (PubMed:9153233, PubMed:9299485).