Target：SAP 14
Fields：Spliceosome
Gene Name：SF3B14
Protein Name：Pre-mRNA branch site protein p14
Human Gene Id：51639
Human Swiss Prot No：Q9Y3B4
Mouse Gene Id：66055
Mouse Swiss Prot No：P59708
Immunogen：The antiserum was produced against synthesized peptide derived from human SF3B14. AA range:76-125
Specificity：SAP 14 Polyclonal Antibody detects endogenous levels of SAP 14 protein.
Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source：Polyclonal, Rabbit,IgG
Dilution：WB 1:500 - 1:2000. ELISA: 1:40000. Not yet tested in other applications.
Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration：1 mg/ml
Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)
Other Name：SF3B14;CGI-110;HSPC175;HT006;Pre-mRNA branch site protein p14;SF3b 14 kDa subunit
Observed Band(KD)：14kD
Background： This gene encodes a 14 kDa protein subunit of the splicing factor 3b complex. Splicing factor 3b associates with both the U2 and U11/U12 small nuclear ribonucleoprotein complexes (U2 snRNP) of spliceosomes. This 14 kDa protein interacts directly with subunit 1 of the splicing factor 3b complex. This 14 kDa protein also interacts directly with the adenosine that carries out the first transesterification step of splicing at the pre-mRNA branch site. [provided by RefSeq, Jul 2008],
Function：function:Necessary for the splicing of pre-mRNA. Directly contacts the pre-mRNA branch site adenosine for the first catalytic step of splicing. Enters the spliceosome and associates with the pre-mRNA branch site as part of the 17S U2 or, in the case of the minor spliceosome, as part of the 18S U11/U12 snRNP complex, and thus may facilitate the interaction of these snRNP with the branch sites of U2 and U12 respectively.,similarity:Contains 1 RRM (RNA recognition motif) domain.,subunit:Interacts with SF3B1/SF3b155 in the region of amino acids 255-424. Interacts to a lesser extent with SF3b130.,
Subcellular Location：Nucleus .
Expression： Hypothalamus,Testis,Umbilical cord blood,Uterus,
