Target：Sam 68

Gene Name：KHDRBS1

Protein Name：KH domain-containing RNA-binding signal transduction-associated protein 1

Human Gene Id：10657

Human Swiss Prot No：Q07666

Mouse Gene Id：20218

Mouse Swiss Prot No：Q60749

Rat Gene Id：117268

Rat Swiss Prot No：Q91V33

Immunogen：The antiserum was produced against synthesized peptide derived from human Sam 68. AA range:96-145

Specificity：Sam 68 Polyclonal Antibody detects endogenous levels of Sam 68 protein.

Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Source：Polyclonal, Rabbit,IgG

Dilution：WB 1:500 - 1:2000. ELISA: 1:10000. Not yet tested in other applications.

Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration：1 mg/ml

Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)

Other Name：KHDRBS1;SAM68;KH domain-containing; RNA-binding, signal transduction-associated protein 1;GAP-associated tyrosine phosphoprotein p62;Src-associated in mitosis 68 kDa protein;Sam68;p21 Ras GTPase-activating protein-associated p62;p68

Observed Band(KD)：68kD

Background： This gene encodes a member of the K homology domain-containing, RNA-binding, signal transduction-associated protein family. The encoded protein appears to have many functions and may be involved in a variety of cellular processes, including alternative splicing, cell cycle regulation, RNA 3'-end formation, tumorigenesis, and regulation of human immunodeficiency virus gene expression. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2012],

Function：developmental stage:Isoform 3 is only expressed in growth-arrested cells.,domain:The KH domain is required for binding to RNA.,domain:The Pro-rich domains are flanked by Arg/Gly-rich motifs which can be asymmetric dimethylated on arginine residues to give the DMA/Gly-rich regions. Selective methlylation on these motifs can modulate protein-protein interactions.,function:Isoform 3, which is expressed in growth-arrested cells only, inhibits S phase.,function:Recruited and tyrosine phosphorylated by several receptor systems, for example the T-cell, leptin and insulin receptors. Once phosphorylated, functions as an adapter protein in signal transduction cascades by binding to SH2 and SH3 domain-containing proteins. Role in G2-M progression in the cell cycle. Represses CBP-dependent transcriptional activation apparently by competing with other nuclear factors for binding to CBP. Also acts as

Subcellular Location：Nucleus . Cytoplasm . Membrane . Predominantly located in the nucleus but also located partially in the cytoplasm. .

Expression：Ubiquitously expressed in all tissue examined. Isoform 1 is expressed at lower levels in brain, skeletal muscle, and liver whereas isoform 3 is intensified in skeletal muscle and in liver.