Target：SAE1

Fields：Ubiquitin mediated proteolysis

Gene Name：SAE1

Protein Name：SUMO-activating enzyme subunit 1

Human Gene Id：10055

Human Swiss Prot No：Q9UBE0

Mouse Gene Id：56459

Mouse Swiss Prot No：Q9R1T2

Rat Gene Id：308384

Rat Swiss Prot No：Q6AXQ0

Immunogen：The antiserum was produced against synthesized peptide derived from human AOS1. AA range:221-270

Specificity：SAE1 Polyclonal Antibody detects endogenous levels of SAE1 protein.

Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Source：Polyclonal, Rabbit,IgG

Dilution：WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.

Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration：1 mg/ml

Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)

Other Name：SAE1;AOS1;SUA1;UBLE1A;SUMO-activating enzyme subunit 1;Ubiquitin-like 1-activating enzyme E1A

Observed Band(KD)：38kD

Background：SUMO1 activating enzyme subunit 1(SAE1) Homo sapiens Posttranslational modification of proteins by the addition of the small protein SUMO (see SUMO1; MIM 601912), or sumoylation, regulates protein structure and intracellular localization. SAE1 and UBA2 (MIM 613295) form a heterodimer that functions as a SUMO-activating enzyme for the sumoylation of proteins (Okuma et al., 1999 [PubMed 9920803]).[supplied by OMIM, Mar 2010],

Function：function:The dimeric enzyme acts as a E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins and formation of a thioester with a conserved cysteine residue on SAE2.,pathway:Protein modification; protein sumoylation.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Belongs to the ubiquitin-activating E1 family.,subunit:Heterodimer of SAE1 and SAE2. The complex binds SUMO proteins via SAE2.,tissue specificity:Expression level increases during S phase and drops in G2 phase (at protein level).,

Subcellular Location：Nucleus .

Expression：Expression level increases during S phase and drops in G2 phase (at protein level).