Target：AKAP 149

Gene Name：AKAP1

Protein Name：A-kinase anchor protein 1 mitochondrial

Human Gene Id：8165

Human Swiss Prot No：Q92667

Mouse Swiss Prot No：O08715

Immunogen：The antiserum was produced against synthesized peptide derived from human AKAP1. AA range:281-330

Specificity：AKAP 149 Polyclonal Antibody detects endogenous levels of AKAP 149 protein.

Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Source：Polyclonal, Rabbit,IgG

Dilution：WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:5000. Not yet tested in other applications.

Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration：1 mg/ml

Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)

Other Name：AKAP1;AKAP149;PRKA1;A-kinase anchor protein 1; mitochondrial;A-kinase anchor protein 149 kDa;AKAP 149;Dual specificity A-kinase-anchoring protein 1;D-AKAP-1;Protein kinase A-anchoring protein 1;PRKA1;Spermatid A-kinase anchor prot

Observed Band(KD)：90kD

Background： The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. The encoded protein binds to type I and type II regulatory subunits of PKA and anchors them to the mitochondrion. This protein is speculated to be involved in the cAMP-dependent signal transduction pathway and in directing RNA to a specific cellular compartment. [provided by RefSeq, Jul 2008],

Function：domain:RII-alpha binding site, predicted to form an amphipathic helix, could participate in protein-protein interactions with a complementary surface on the R-subunit dimer.,function:Binds to type I and II regulatory subunits of protein kinase A and anchors them to the cytoplasmic face of the mitochondrial outer membrane.,similarity:Contains 1 KH domain.,similarity:Contains 1 Tudor domain.,tissue specificity:AKAP149 is highly expressed in prostate and small intestine whereas S-AKAP84 is expressed in kidney, pancreas, liver, lung and brain. AKAP149 is also expressed in colon carcinoma.,

Subcellular Location：Mitochondrion outer membrane . Mitochondrion .

Expression：Isoform 1 is detected in thymus, prostate, testis, ovary, colon and small intestine (PubMed:8769136). Isoform 2 is highly expressed in testis and detected at much lower levels in kidney, pancreas, liver, lung and brain (PubMed:7499250).