Target：GPT2

Fields：Arginine biosynthesis;Alanine, aspartate and glutamate metabolism;Metabolic pathways;Carbon metabolism;2-Oxocarboxylic acid metabolism;Biosynthesis of amino acids

Gene Name：GPT2

Protein Name：Alanine aminotransferase 2

Human Gene Id：84706

Human Swiss Prot No：Q8TD30

Mouse Swiss Prot No：Q8BGT5

Immunogen：Synthesized peptide derived from GPT2 . at AA range: 190-270

Specificity：GPT2 Polyclonal Antibody detects endogenous levels of GPT2 protein.

Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Source：Polyclonal, Rabbit,IgG

Dilution：WB 1:500 - 1:2000. ELISA: 1:40000. Not yet tested in other applications.

Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration：1 mg/ml

Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)

Other Name：GPT2;AAT2;ALT2;Alanine aminotransferase 2;ALT2;Glutamate pyruvate transaminase 2;GPT 2;Glutamic--alanine transaminase 2;Glutamic--pyruvic transaminase 2

Observed Band(KD)：50kD

Background： This gene encodes a mitochondrial alanine transaminase, a pyridoxal enzyme that catalyzes the reversible transamination between alanine and 2-oxoglutarate to generate pyruvate and glutamate. Alanine transaminases play roles in gluconeogenesis and amino acid metabolism in many tissues including skeletal muscle, kidney, and liver. Activating transcription factor 4 upregulates this gene under metabolic stress conditions in hepatocyte cell lines. A loss of function mutation in this gene has been associated with developmental encephalopathy. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2015],

Function：catalytic activity:L-alanine + 2-oxoglutarate = pyruvate + L-glutamate.,cofactor:Pyridoxal phosphate.,pathway:Amino-acid degradation; L-alanine degradation via transaminase pathway; pyruvate from L-alanine: step 1/1.,similarity:Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. Alanine aminotransferase subfamily.,subunit:Homodimer.,tissue specificity:Expressed at high levels in muscle, adipose tissue, kidney and brain and at lower levels in the liver and breast.,

Subcellular Location：mitochondrion,mitochondrial matrix,

Expression：Expressed at high levels in muscle, adipose tissue, kidney and brain and at lower levels in the liver and breast.