Target：LARG

Fields：Vascular smooth muscle contraction;Axon guidance;Platelet activation;C-type lectin receptor signaling pathway;Regulation of actin cytoskeleton;Pathogenic Escherichia coli infection;Yersinia infection;Tuberculosis;Human cytomegalovirus infection;Pathways in cancer;Proteoglycans in cancer

Gene Name：ARHGEF12

Protein Name：Rho guanine nucleotide exchange factor 12

Human Gene Id：23365

Human Swiss Prot No：Q9NZN5

Mouse Swiss Prot No：Q8R4H2

Immunogen：The antiserum was produced against synthesized peptide derived from human ARHGEF12. AA range:449-498

Specificity：LARG Polyclonal Antibody detects endogenous levels of LARG protein.

Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Source：Polyclonal, Rabbit,IgG

Dilution：WB 1:500-2000;IHC 1:50-300

Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration：1 mg/ml

Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)

Other Name：ARHGEF12;KIAA0382;LARG;Rho guanine nucleotide exchange factor 12;Leukemia-associated RhoGEF

Observed Band(KD)：173kD

Background： Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli working through G protein-coupled receptors. The encoded protein may form a complex with G proteins and stimulate Rho-dependent signals. This protein has been observed to form a myeloid/lymphoid fusion partner in acute myeloid leukemia. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2014],

Function：disease:A chromosomal aberration involving ARHGEF12 may be a cause of acute leukemia. Translocation t(11;11)(q23;23) with MLL.,function:May play a role in the regulation of RhoA GTPase by guanine nucleotide-binding alpha-12 (GNA12) and alpha-13 (GNA13). Acts as guanine nucleotide exchange factor (GEF) for RhoA GTPase and may act as GTPase-activating protein (GAP) for GNA12 and GNA13.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,sequence caution:Contaminating sequence. Potential poly-A sequence.,similarity:Contains 1 DH (DBL-homology) domain.,similarity:Contains 1 PDZ (DHR) domain.,similarity:Contains 1 PH domain.,similarity:Contains 1 RGSL (RGS-like) domain.,subcellular location:Translocated to the membrane upon stimulation.,subunit:Interacts with GNA12 and GNA13, probably through the RGS-like domain. Interacts with RHOA, PLXNB1 and PLXNB2. Interacts through its PDZ domain

Subcellular Location：Cytoplasm . Membrane . Translocated to the membrane upon stimulation. .

Expression：Ubiquitously expressed. Isoform 2 is found in jejunum and testis.