Target：RPA135
Fields：RNA polymerase
Gene Name：POLR1B
Protein Name：DNA-directed RNA polymerase I subunit RPA2
Human Gene Id：84172
Human Swiss Prot No：Q9H9Y6
Mouse Gene Id：20017
Mouse Swiss Prot No：P70700
Rat Gene Id：83582
Rat Swiss Prot No：O54888
Immunogen：Synthesized peptide derived from the Internal region of human RPA135.
Specificity：RPA135 Polyclonal Antibody detects endogenous levels of RPA135 protein.
Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source：Polyclonal, Rabbit,IgG
Dilution：WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:40000.. IF 1:50-200
Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration：1 mg/ml
Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)
Other Name：POLR1B;DNA-directed RNA polymerase I subunit RPA2;RNA polymerase I subunit 2;DNA-directed RNA polymerase I 135 kDa polypeptide;RPA135
Observed Band(KD)：125kD
Background： Eukaryotic RNA polymerase I (pol I) is responsible for the transcription of ribosomal RNA (rRNA) genes and production of rRNA, the primary component of ribosomes. Pol I is a multisubunit enzyme composed of 6 to 14 polypeptides, depending on the species. Most of the mass of the pol I complex derives from the 2 largest subunits, Rpa1 and Rpa2 in yeast. POLR1B is homologous to Rpa2 (Seither and Grummt, 1996 [PubMed 8921381]).[supplied by OMIM, Mar 2008],
Function：catalytic activity:Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).,function:DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Second largest core component of RNA polymerase I which synthesizes ribosomal RNA precursors. Proposed to contribute to the polymerase catalytic activity and forms the polymerase active center together with the largest subunit. Pol I is composed of mobile elements and RPA2 is part of the core element with the central large cleft and probably a clamp element that moves to open and close the cleft.,similarity:Belongs to the RNA polymerase beta chain family.,subunit:Component of the RNA polymerase I (Pol I) complex consisting of at least 13 subunits.,
Subcellular Location：Nucleus, nucleolus . Chromosome .
Expression： Eye,Pancreas,Skin,Testis,
