Target:Cdc34
Fields:Ubiquitin mediated proteolysis
Gene Name:CDC34
Protein Name:Ubiquitin-conjugating enzyme E2 R1
Human Gene Id:997
Human Swiss Prot No:P49427
Mouse Gene Id:216150
Mouse Swiss Prot No:Q8CFI2
Immunogen:The antiserum was produced against synthesized peptide derived from the Internal region of human CDC34. AA range:121-170
Specificity:Cdc34 Polyclonal Antibody detects endogenous levels of Cdc34 protein.
Formulation:Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source:Polyclonal, Rabbit,IgG
Dilution:WB 1:500 - 1:2000. IHC: 1:100-300 ELISA: 1:20000.. IF 1:50-200
Purification:The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration:1 mg/ml
Storage Stability:-15°C to -25°C/1 year(Do not lower than -25°C)
Other Name:CDC34;UBCH3;UBE2R1;Ubiquitin-conjugating enzyme E2 R1;Ubiquitin-conjugating enzyme E2-32 kDa complementing;Ubiquitin-conjugating enzyme E2-CDC34;Ubiquitin-protein ligase R1
Observed Band(KD):34kD
Background: The protein encoded by this gene is a member of the ubiquitin-conjugating enzyme family. Ubiquitin-conjugating enzyme catalyzes the covalent attachment of ubiquitin to other proteins. This protein is a part of the large multiprotein complex, which is required for ubiquitin-mediated degradation of cell cycle G1 regulators, and for the initiation of DNA replication. [provided by RefSeq, Jul 2008],
Function:catalytic activity:ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.,function:Catalyzes the covalent attachment of ubiquitin to other proteins. May be involved in degradation of katenin.,pathway:Protein modification; protein ubiquitination.,similarity:Belongs to the ubiquitin-conjugating enzyme family.,subunit:Interacts with SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complex. When phosphorylated, interacts with beta-TrCP (BTRC).,
Subcellular Location:Cytoplasm. Nucleus. The phosphorylation of the C-terminal tail plays an important role in mediating nuclear localization. Colocalizes with beta-tubulin on mitotic spindles in anaphase.
Expression:Expressed in testes during spermatogenesis to regulate repression of cAMP-induced transcription.