Target：Cdc27

Fields：Cell cycle;Oocyte meiosis;Ubiquitin mediated proteolysis;Progesterone-mediated oocyte maturation;Human T-cell leukemia virus 1 infection

Gene Name：CDC27

Protein Name：Cell division cycle protein 27 homolog

Human Gene Id：996

Human Swiss Prot No：P30260

Mouse Gene Id：217232

Mouse Swiss Prot No：A2A6Q5

Rat Gene Id：360643

Rat Swiss Prot No：Q4V8A2

Immunogen：The antiserum was produced against synthesized peptide derived from human H-NUC. AA range:361-410

Specificity：Cdc27 Polyclonal Antibody detects endogenous levels of Cdc27 protein.

Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Source：Polyclonal, Rabbit,IgG

Dilution：WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:5000.. IF 1:50-200

Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration：1 mg/ml

Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)

Other Name：CDC27;ANAPC3;D0S1430E;D17S978E;Cell division cycle protein 27 homolog;Anaphase-promoting complex subunit 3;APC3;CDC27 homolog;CDC27Hs;H-NUC

Observed Band(KD)：92kD

Background： The protein encoded by this gene shares strong similarity with Saccharomyces cerevisiae protein Cdc27, and the gene product of Schizosaccharomyces pombe nuc 2. This protein is a component of the anaphase-promoting complex (APC), which is composed of eight protein subunits and is highly conserved in eukaryotic cells. This complex catalyzes the formation of cyclin B-ubiquitin conjugate, which is responsible for the ubiquitin-mediated proteolysis of B-type cyclins. The protein encoded by this gene and three other members of the APC complex contain tetratricopeptide (TPR) repeats, which are important for protein-protein interactions. This protein was shown to interact with mitotic checkpoint proteins including Mad2, p55CDC and BUBR1, and it may thus be involved in controlling the timing of mitosis. Alternative splicing of this gene results in multiple transcript variants. Related pseudogenes have been i

Function：PTM:Phosphorylated. Phosphorylation on Ser-426 and Thr-446 occurs specifically during mitosis.,similarity:Belongs to the APC3/CDC27 family.,similarity:Contains 9 TPR repeats.,subunit:Interacts with RB.,

Subcellular Location：Nucleus . Cytoplasm, cytoskeleton, spindle .

Expression： Epithelium,Uterus,