Target：CaMKK2

Fields：Autophagy - animal;AMPK signaling pathway;Longevity regulating pathway;Adipocytokine signaling pathway;Oxytocin signaling pathway;Alcoholic liver disease;Alcoholism

Gene Name：CAMKK2

Protein Name：Calcium/calmodulin-dependent protein kinase kinase 2

Human Gene Id：10645

Human Swiss Prot No：Q96RR4

Mouse Gene Id：207565

Mouse Swiss Prot No：Q8C078

Rat Gene Id：83506

Rat Swiss Prot No：O88831

Immunogen：The antiserum was produced against synthesized peptide derived from human CAMKK2. AA range:381-430

Specificity：CaMKIIβ Polyclonal Antibody detects endogenous levels of CaMKIIβ protein.

Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Source：Polyclonal, Rabbit,IgG

Dilution：WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:10000.. IF 1:50-200

Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration：1 mg/ml

Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)

Other Name：CAMKK2;CAMKKB;KIAA0787;Calcium/calmodulin-dependent protein kinase kinase 2;CaM-KK 2;CaM-kinase kinase 2;CaMKK 2;Calcium/calmodulin-dependent protein kinase kinase beta;CaM-KK beta;CaM-kinase kinase beta;CaMKK beta

Observed Band(KD)：65kD

Background： The product of this gene belongs to the Serine/Threonine protein kinase family, and to the Ca(2+)/calmodulin-dependent protein kinase subfamily. The major isoform of this gene plays a role in the calcium/calmodulin-dependent (CaM) kinase cascade by phosphorylating the downstream kinases CaMK1 and CaMK4. Protein products of this gene also phosphorylate AMP-activated protein kinase (AMPK). This gene has its strongest expression in the brain and influences signalling cascades involved with learning and memory, neuronal differentiation and migration, neurite outgrowth, and synapse formation. Alternative splicing results in multiple transcript variants encoding distinct isoforms. The identified isoforms differ in their ability to undergo autophosphorylation and to phosphorylate downstream kinases. [provided by RefSeq, Jul 2012],

Function：catalytic activity:ATP + a protein = ADP + a phosphoprotein.,domain:The autoinhibitory domain overlaps with the calmodulin binding region and may be involved in intrasteric autoinhibition.,domain:The RP domain (arginine/proline-rich) is involved in the recognition of CAMKI and CAMK4 as substrates.,enzyme regulation:Activated by Ca(2+)/calmodulin. Binding of calmodulin may releave intrasteric autoinhibition. Autophosphorylation does not alter activity or regulation by Ca(2+)/calmodulin. In part, activity is independent on Ca(2+)/calmodulin.,function:Calcium/calmodulin-dependent protein kinase belonging to a proposed calcium-triggered signaling cascade involved in a number of cellular processes. Isoform 1, isoform 2 and isoform 3 phosphorylate CAMK1 and CAMK4. Isoform 3 phosphorylates CAMK1D. Isoform 4, isoform 5 and isoform 6 lacking part of the calmodulin-binding domain are inactive. See

Subcellular Location：Nucleus . Cytoplasm . Cell projection, neuron projection . Predominantly nuclear in unstimulated cells, relocalizes into cytoplasm and neurites after forskolin induction. .

Expression：Ubiquitously expressed with higher levels in the brain. Intermediate levels are detected in spleen, prostate, thyroid and leukocytes. The lowest level is in lung.