Target：Gl Syn

Fields：Arginine biosynthesis;Alanine, aspartate and glutamate metabolism;Glyoxylate and dicarboxylate metabolism;Nitrogen metabolism;Metabolic pathways;Biosynthesis of amino acids;Necroptosis;Glutamatergic synapse;GABAergic synapse

Gene Name：GLUL

Protein Name：Glutamine synthetase

Human Gene Id：2752

Human Swiss Prot No：P15104

Mouse Gene Id：14645

Mouse Swiss Prot No：P15105

Rat Gene Id：24957

Rat Swiss Prot No：P09606

Immunogen：The antiserum was produced against synthesized peptide derived from human Gl Syn. AA range:295-344

Specificity：Gl Syn Polyclonal Antibody detects endogenous levels of Gl Syn protein.

Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Source：Polyclonal, Rabbit,IgG

Dilution：WB 1:500 - 1:2000. ELISA: 1:20000. Not yet tested in other applications.

Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration：1 mg/ml

Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)

Other Name：GLUL;GLNS;Glutamine synthetase;GS;Glutamate decarboxylase;Glutamate--ammonia ligase

Observed Band(KD)：42kD

Background： The protein encoded by this gene belongs to the glutamine synthetase family. It catalyzes the synthesis of glutamine from glutamate and ammonia in an ATP-dependent reaction. This protein plays a role in ammonia and glutamate detoxification, acid-base homeostasis, cell signaling, and cell proliferation. Glutamine is an abundant amino acid, and is important to the biosynthesis of several amino acids, pyrimidines, and purines. Mutations in this gene are associated with congenital glutamine deficiency, and overexpression of this gene was observed in some primary liver cancer samples. There are six pseudogenes of this gene found on chromosomes 2, 5, 9, 11, and 12. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014],

Function：catalytic activity:ATP + L-glutamate + NH(3) = ADP + phosphate + L-glutamine.,disease:Defects in GLUL are the cause of congenital systemic glutamine deficiency (CSGD) [MIM:610015]. CSGD is a rare developmental disorder with severe brain malformation resulting in multi-organ failure and neonatal death. Glutamine is largely absent from affected patients serum, urine and cerebrospinal fluid.,online information:Glutamine synthetase entry,similarity:Belongs to the glutamine synthetase family.,subunit:Homooctamer.,

Subcellular Location：Cytoplasm, cytosol . Microsome . Mitochondrion . Cell membrane ; Lipid-anchor . Mainly localizes in the cytosol, with a fraction associated with the cell membrane. .

Expression：Expressed in endothelial cells.