Target：ME2

Fields：Pyruvate metabolism;Carbon metabolism

Gene Name：ME2

Protein Name：NAD-dependent malic enzyme mitochondrial

Human Gene Id：4200

Human Swiss Prot No：P23368

Mouse Swiss Prot No：Q99KE1

Immunogen：The antiserum was produced against synthesized peptide derived from human ME2. AA range:201-250

Specificity：ME2 Polyclonal Antibody detects endogenous levels of ME2 protein.

Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Source：Polyclonal, Rabbit,IgG

Dilution：WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:40000.. IF 1:50-200

Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration：1 mg/ml

Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)

Other Name：ME2;NAD-dependent malic enzyme; mitochondrial;NAD-ME;Malic enzyme 2

Observed Band(KD)：65kD

Background： This gene encodes a mitochondrial NAD-dependent malic enzyme, a homotetrameric protein, that catalyzes the oxidative decarboxylation of malate to pyruvate. It had previously been weakly linked to a syndrome known as Friedreich ataxia that has since been shown to be the result of mutation in a completely different gene. Certain single-nucleotide polymorphism haplotypes of this gene have been shown to increase the risk for idiopathic generalized epilepsy. Alternatively spliced transcript variants encoding different isoforms found for this gene. [provided by RefSeq, Dec 2009],

Function：catalytic activity:(S)-malate + NAD(+) = pyruvate + CO(2) + NADH.,cofactor:Divalent metal cations. Prefers magnesium or manganese.,enzyme regulation:Subject to allosteric activation by fumarate.,miscellaneous:This isoenzyme can also use NADP(+) but is more effective with NAD(+).,similarity:Belongs to the malic enzymes family.,subunit:Homotetramer.,

Subcellular Location：Mitochondrion matrix .

Expression： B-cell,Placenta,Platelet,