Target：POLR2E

Fields：RNA polymerase;Cytosolic DNA-sensing pathway;Huntington disease

Gene Name：POLR2E

Protein Name：DNA-directed RNA polymerases I, II, and III subunit RPABC1

Human Gene Id：5434

Human Swiss Prot No：P19388

Mouse Gene Id：66420

Mouse Swiss Prot No：Q80UW8

Rat Gene Id：690966

Rat Swiss Prot No：B0BNE2

Immunogen：The antiserum was produced against synthesized peptide derived from human RPAB1. AA range:21-70

Specificity：POLR2E Polyclonal Antibody detects endogenous levels of POLR2E protein.

Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Source：Polyclonal, Rabbit,IgG

Dilution：WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:40000.. IF 1:50-200

Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration：1 mg/ml

Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)

Other Name：POLR2E;DNA-directed RNA polymerases I; II, and III subunit RPABC1;RNA polymerases I, II, and III subunit ABC1;DNA-directed RNA polymerase II 23 kDa polypeptide;DNA-directed RNA polymerase II subunit E;RPB5 homolog;XAP4

Observed Band(KD)：25kD

Background： This gene encodes the fifth largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit is shared by the other two DNA-directed RNA polymerases and is present in two-fold molar excess over the other polymerase subunits. An interaction between this subunit and a hepatitis virus transactivating protein has been demonstrated, suggesting that interaction between transcriptional activators and the polymerase can occur through this subunit. A pseudogene is located on chromosome 11. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Oct 2015],

Function：function:DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Common component of RNA polymerases I, II and III which synthesize ribosomal RNA precursors, mRNA precursors and many functional non-coding RNAs, and small RNAs, such as 5S rRNA and tRNAs, respectively. Pol II is the central component of the basal RNA polymerase II transcription machinery. Pols are composed of mobile elements that move relative to each other. In Pol II, POLR2E/RPB5 is part of the lower jaw surrounding the central large cleft and thought to grab the incoming DNA template. Seems to be the major component in this process.,PTM:The N-terminus is blocked.,similarity:Belongs to the archaeal rpoH/eukaryotic RPB5 RNA polymerase subunit family.,subunit:Component of the RNA polymerase I (Pol I), RNA polymerase II (Pol II) and RNA polymerase II

Subcellular Location：Nucleus .

Expression： Lung,