Target：Ephrin-A1

Fields：MAPK signaling pathway;Ras signaling pathway;Rap1 signaling pathway;PI3K-Akt signaling pathway;Axon guidance;MicroRNAs in cancer

Gene Name：EFNA1

Protein Name：Ephrin-A1

Human Gene Id：1942

Human Swiss Prot No：P20827

Mouse Gene Id：13636

Mouse Swiss Prot No：P52793

Rat Gene Id：94268

Rat Swiss Prot No：P97553

Immunogen：The antiserum was produced against synthesized peptide derived from human EFNA1. AA range:66-115

Specificity：Ephrin-A1 Polyclonal Antibody detects endogenous levels of Ephrin-A1 protein.

Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Source：Polyclonal, Rabbit,IgG

Dilution：WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:10000. Not yet tested in other applications.

Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration：1 mg/ml

Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)

Other Name：EFNA1;EPLG1;LERK1;TNFAIP4;Ephrin-A1;EPH-related receptor tyrosine kinase ligand 1;LERK-1;Immediate early response protein B61;Tumor necrosis factor alpha-induced protein 4;TNF alpha-induced protein 4

Observed Band(KD)：24kD

Background： This gene encodes a member of the ephrin (EPH) family. The ephrins and EPH-related receptors comprise the largest subfamily of receptor protein-tyrosine kinases and have been implicated in mediating developmental events, especially in the nervous system and in erythropoiesis. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. This gene encodes an EFNA class ephrin which binds to the EPHA2, EPHA4, EPHA5, EPHA6, and EPHA7 receptors. Two transcript variants that encode different isoforms were identified through sequence analysis. [provided by RefSeq, Jul 2008],

Function：induction:By TNF-alpha and interleukin-1 beta.,similarity:Belongs to the ephrin family.,subunit:Binds to the receptor tyrosine kinases EPHA2, EPHA4, EPHA5, EPHA6 and EPHA7. Also binds with low affinity to EPHA1.,

Subcellular Location：Cell membrane ; Lipid-anchor, GPI-anchor .; [Ephrin-A1, secreted form]: Secreted .

Expression：Brain. Down-regulated in primary glioma tissues compared to the normal tissues. The soluble monomeric form is expressed in the glioblastoma multiforme (GBM) and breast cancer cells (at protein level).