Target：Epac

Fields：Rap1 signaling pathway;cAMP signaling pathway;Phospholipase D signaling pathway;Adrenergic signaling in cardiomyocytes;Leukocyte transendothelial migration;Long-term potentiation;Serotonergic synapse

Gene Name：RAPGEF3

Protein Name：Rap guanine nucleotide exchange factor 3

Human Gene Id：10411

Human Swiss Prot No：O95398

Mouse Gene Id：223864

Mouse Swiss Prot No：Q8VCC8

Rat Gene Id：59326

Rat Swiss Prot No：Q9Z1C8

Immunogen：The antiserum was produced against synthesized peptide derived from human RAPGEF3. AA range:712-761

Specificity：Epac Polyclonal Antibody detects endogenous levels of Epac protein.

Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Source：Polyclonal, Rabbit,IgG

Dilution：WB 1:500 - 1:2000. ELISA: 1:40000. Not yet tested in other applications.

Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration：1 mg/ml

Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)

Other Name：RAPGEF3;CGEF1;EPAC;EPAC1;Rap guanine nucleotide exchange factor 3;Exchange factor directly activated by cAMP 1;Exchange protein directly activated by cAMP 1;EPAC 1;Rap1 guanine-nucleotide-exchange factor directly activated by cAMP;

Observed Band(KD)：100kD

Background：caution:It is uncertain whether Met-1 or Met-43 is the initiator.,domain:The DEP domain is involved in membrane localization independent from regulation by cAMP.,function:Guanine nucleotide exchange factor (GEF) for RAP1A and RAP2A small GTPases that is activated by binding cAMP.,similarity:Contains 1 cyclic nucleotide-binding domain.,similarity:Contains 1 DEP domain.,similarity:Contains 1 N-terminal Ras-GEF domain.,similarity:Contains 1 Ras-GEF domain.,tissue specificity:Widely expressed with highest levels in adult kidney, heart, thyroid and brain, and fetal kidney.,

Function：caution:It is uncertain whether Met-1 or Met-43 is the initiator.,domain:The DEP domain is involved in membrane localization independent from regulation by cAMP.,function:Guanine nucleotide exchange factor (GEF) for RAP1A and RAP2A small GTPases that is activated by binding cAMP.,similarity:Contains 1 cyclic nucleotide-binding domain.,similarity:Contains 1 DEP domain.,similarity:Contains 1 N-terminal Ras-GEF domain.,similarity:Contains 1 Ras-GEF domain.,tissue specificity:Widely expressed with highest levels in adult kidney, heart, thyroid and brain, and fetal kidney.,

Subcellular Location：Endomembrane system .

Expression：Widely expressed with highest levels in adult kidney, heart, thyroid and brain, and fetal kidney.