Target：eIF5B

Gene Name：EIF5B

Protein Name：Eukaryotic translation initiation factor 5B

Human Gene Id：9669

Human Swiss Prot No：O60841

Mouse Gene Id：226982

Mouse Swiss Prot No：Q05D44

Rat Gene Id：308306

Rat Swiss Prot No：B2GUV7

Immunogen：The antiserum was produced against synthesized peptide derived from human EIF5B. AA range:1051-1100

Specificity：eIF5B Polyclonal Antibody detects endogenous levels of eIF5B protein.

Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Source：Polyclonal, Rabbit,IgG

Dilution：WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:40000.. IF 1:50-200

Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration：1 mg/ml

Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)

Other Name：EIF5B;IF2;KIAA0741;Eukaryotic translation initiation factor 5B;eIF-5B;Translation initiation factor IF-2

Observed Band(KD)：139kD

Background： Accurate initiation of translation in eukaryotes is complex and requires many factors, some of which are composed of multiple subunits. The process is simpler in prokaryotes which have only three initiation factors (IF1, IF2, IF3). Two of these factors are conserved in eukaryotes: the homolog of IF1 is eIF1A and the homolog of IF2 is eIF5B. This gene encodes eIF5B. Factors eIF1A and eIF5B interact on the ribosome along with other initiation factors and GTP to position the initiation methionine tRNA on the start codon of the mRNA so that translation initiates accurately. [provided by RefSeq, Jul 2008],

Function：function:Function in general translation initiation by promoting the binding of the formylmethionine-tRNA to ribosomes. Seems to function along with eIF-2.,similarity:Belongs to the IF-2 family.,subunit:Interacts with ANXA5 in a calcium and phospholipid-dependent manner.,

Subcellular Location：Cytoplasm .

Expression： Brain,Cervix carcinoma,Epithelium,Hepatocyte,Liver,Lymph,Pl