Target：ATBF1

Fields：Signaling pathways regulating pluripotency of stem cells

Gene Name：ZFHX3

Protein Name：Zinc finger homeobox protein 3

Human Gene Id：463

Human Swiss Prot No：Q15911

Mouse Swiss Prot No：Q61329

Immunogen：The antiserum was produced against synthesized peptide derived from human ZFHX3. AA range:761-810

Specificity：ATBF1 Polyclonal Antibody detects endogenous levels of ATBF1 protein.

Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Source：Polyclonal, Rabbit,IgG

Dilution：IHC 1:100 - 1:300. ELISA: 1:10000.. IF 1:50-200

Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration：1 mg/ml

Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)

Other Name：ZFHX3;ATBF1;Zinc finger homeobox protein 3;AT motif-binding factor;AT-binding transcription factor 1;Alpha-fetoprotein enhancer-binding protein;Zinc finger homeodomain protein 3;ZFH-3

Molecular Weight(Da)：404kD

Background： This gene encodes a transcription factor with multiple homeodomains and zinc finger motifs, and regulates myogenic and neuronal differentiation. The encoded protein suppresses expression of the alpha-fetoprotein gene by binding to an AT-rich enhancer motif. The protein has also been shown to negatively regulate c-Myb, and transactivate the cell cycle inhibitor cyclin-dependent kinase inhibitor 1A (also known as p21CIP1). This gene is reported to function as a tumor suppressor in several cancers, and sequence variants of this gene are also associated with atrial fibrillation. Multiple transcript variants expressed from alternate promoters and encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2009],

Function：function:Transcriptional activator that binds to the AT-rich core sequence of the enhancer element of the AFP gene.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Contains 22 C2H2-type zinc fingers.,similarity:Contains 4 homeobox DNA-binding domains.,subunit:Interacts with FNBP3.,

Subcellular Location：Nucleus . Cytoplasm . Translocates from the cytoplasm to the nucleus following TGF-beta stimulation. Expressed in nuclear body (NB)-like dots in the nucleus some of which overlap or closely associate with PML body. .

Expression：Not found in normal gastric mucosa but found in gastric carcinoma cells (at protein level). Expression is higher in ER-positive breast tumors than ER-negative breast tumors (at protein level).