Target：ECA39

Fields：Cysteine and methionine metabolism;Valine, leucine and isoleucine degradation;Valine, leucine and isoleucine biosynthesis;Pantothenate and CoA biosynthesis;Metabolic pathways;2-Oxocarboxylic acid metabolism;Biosynthesis of amino acids;Biosynthesis of cofactors

Gene Name：BCAT1

Protein Name：Branched-chain-amino-acid aminotransferase, cytosolic

Human Gene Id：586

Human Swiss Prot No：P54687

Mouse Gene Id：12035

Mouse Swiss Prot No：P24288

Rat Gene Id：29592

Rat Swiss Prot No：P54690

Immunogen：The antiserum was produced against synthesized peptide derived from the Internal region of human BCAT1. AA range:231-280

Specificity：ECA39 Polyclonal Antibody detects endogenous levels of ECA39 protein.

Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Source：Polyclonal, Rabbit,IgG

Dilution：WB 1:500 - 1:2000. IHC: 1:100-1:300. ELISA: 1:20000.. IF 1:50-200

Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration：1 mg/ml

Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)

Other Name：BCAT1;BCT1;ECA39;Branched-chain-amino-acid aminotransferase, cytosolic;BCAT(c);Protein ECA39

Observed Band(KD)：43kD

Background：branched chain amino acid transaminase 1(BCAT1) Homo sapiens This gene encodes the cytosolic form of the enzyme branched-chain amino acid transaminase. This enzyme catalyzes the reversible transamination of branched-chain alpha-keto acids to branched-chain L-amino acids essential for cell growth. Two different clinical disorders have been attributed to a defect of branched-chain amino acid transamination: hypervalinemia and hyperleucine-isoleucinemia. As there is also a gene encoding a mitochondrial form of this enzyme, mutations in either gene may contribute to these disorders. Alternatively spliced transcript variants have been described. [provided by RefSeq, May 2010],

Function：catalytic activity:2-oxoglutaric acid + L-isoleucine = (S)-3-methyl-2-oxopentanoic acid + L-glutamic acid.,catalytic activity:2-oxoglutaric acid + L-valine = 3-methyl-2-oxobutanoic acid + L-glutamic acid.,catalytic activity:L-leucine + 2-oxoglutarate = 4-methyl-2-oxopentanoate + L-glutamate.,cofactor:Pyridoxal phosphate.,function:Catalyzes the first reaction in the catabolism of the essential branched chain amino acids leucine, isoleucine, and valine.,similarity:Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family.,subunit:Homodimer.,tissue specificity:During embryogenesis, expressed in the brain and kidney. Overexpressed in C-myc induced tumors such as Burkitt's lymphoma.,

Subcellular Location：Cytoplasm.

Expression：During embryogenesis, expressed in the brain and kidney. Overexpressed in MYC-induced tumors such as Burkitt's lymphoma.