Target：GCSm-γ
Fields：Cysteine and methionine metabolism;Glutathione metabolism;Metabolic pathways;Biosynthesis of cofactors;Ferroptosis
Gene Name：GCLM
Protein Name：Glutamate--cysteine ligase regulatory subunit
Human Gene Id：2730
Human Swiss Prot No：P48507
Mouse Gene Id：14630
Mouse Swiss Prot No：O09172
Rat Gene Id：29739
Rat Swiss Prot No：P48508
Immunogen：The antiserum was produced against synthesized peptide derived from human GCSm-gamma. AA range:42-91
Specificity：GCSm-γ Polyclonal Antibody detects endogenous levels of GCSm-γ protein.
Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source：Polyclonal, Rabbit,IgG
Dilution：WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:10000.. IF 1:50-200
Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration：1 mg/ml
Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)
Other Name：GCLM;GLCLR;Glutamate--cysteine ligase regulatory subunit;GCS light chain;Gamma-ECS regulatory subunit;Gamma-glutamylcysteine synthetase regulatory subunit;Glutamate--cysteine ligase modifier subunit
Observed Band(KD)：31kD
Background： Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase, is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. Gamma glutamylcysteine synthetase deficiency has been implicated in some forms of hemolytic anemia. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Apr 2015],
Function：pathway:Sulfur metabolism; glutathione biosynthesis; glutathione from L-cysteine and L-glutamate: step 1/2.,similarity:Belongs to the aldo/keto reductase family. Glutamate--cysteine ligase light chain subfamily.,subunit:Heterodimer of a catalytic heavy chain and a regulatory light chain.,tissue specificity:In all tissues examined. Highest levels in skeletal muscle.,
Subcellular Location：cytosol,glutamate-cysteine ligase complex,
Expression：In all tissues examined. Highest levels in skeletal muscle.
