Target：APAF1

Fields：Platinum drug resistance;p53 signaling pathway;Apoptosis;Apoptosis - multiple species;Alzheimer disease;Parkinson disease;Amyotrophic lateral sclerosis;Huntington disease;Prion disease;Pathways of neurodegeneration - multiple diseases;Legionellosis;Tuberculosis;Hepatitis C;Hepatitis B;Measles;Influenza A;Herpes simplex virus 1 infection;Epstein-Barr virus infection;Pathways in cancer;Small cell lung cancer;Lipid and atherosclerosis

Gene Name：APAF1

Protein Name：Apoptotic protease-activating factor 1

Human Gene Id：317

Human Swiss Prot No：O14727-6

Immunogen：The antiserum was produced against synthesized peptide derived from human APAF-1-ALT. AA range:289-338

Specificity：Apaf-1-ALT Polyclonal Antibody detects endogenous levels of Apaf-1-ALT protein.

Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Source：Polyclonal, Rabbit,IgG

Dilution：WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:10000. Not yet tested in other applications.

Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration：1 mg/ml

Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)

Other Name：APAF1-interacting protein;APIP;APIP2;CGI-29 protein;MMRP19;dJ179L10.2;likely ortholog of mouse monocyte macrophage 19

Observed Band(KD)：27kD

Background： This gene encodes a cytoplasmic protein that initiates apoptosis. This protein contains several copies of the WD-40 domain, a caspase recruitment domain (CARD), and an ATPase domain (NB-ARC). Upon binding cytochrome c and dATP, this protein forms an oligomeric apoptosome. The apoptosome binds and cleaves caspase 9 preproprotein, releasing its mature, activated form. Activated caspase 9 stimulates the subsequent caspase cascade that commits the cell to apoptosis. Alternative splicing results in several transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008],

Function：domain:The CARD domain mediates interaction with APIP.,function:Oligomeric Apaf-1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis. This activation requires ATP. Isoform 6 is less effective in inducing apoptosis.,induction:By E2F and p53 in apoptotic neurons.,similarity:Contains 1 CARD domain.,similarity:Contains 1 NB-ARC domain.,similarity:Contains 13 WD repeats.,subunit:Monomer. Oligomerizes upon binding of cytochrome c and dATP. Oligomeric Apaf-1 and pro-caspase-9 bind to each other via their respective NH2-terminal CARD domains and consecutively mature caspase-9 is released from the complex. Pro-caspase-3 is recruited into the Apaf-1-pro-caspase-9 complex via interaction with pro-caspase-9. Interacts with APIP.,tissue specificity:Ubiquitous. Highest levels of expression in adult spleen and per

Subcellular Location：Cytoplasm .

Expression：Ubiquitous. Highest levels of expression in adult spleen and peripheral blood leukocytes, and in fetal brain, kidney and lung. Isoform 1 is expressed in heart, kidney and liver.