Target：APAF1

Fields：Platinum drug resistance;p53 signaling pathway;Apoptosis;Apoptosis - multiple species;Alzheimer disease;Parkinson disease;Amyotrophic lateral sclerosis;Huntington disease;Prion disease;Pathways of neurodegeneration - multiple diseases;Legionellosis;Tuberculosis;Hepatitis C;Hepatitis B;Measles;Influenza A;Herpes simplex virus 1 infection;Epstein-Barr virus infection;Pathways in cancer;Small cell lung cancer;Lipid and atherosclerosis

Gene Name：APAF1

Protein Name：Apoptotic protease-activating factor 1

Human Gene Id：317

Human Swiss Prot No：O14727

Mouse Gene Id：11783

Mouse Swiss Prot No：O88879

Rat Gene Id：78963

Rat Swiss Prot No：Q9EPV5

Immunogen：The antiserum was produced against synthesized peptide derived from the Internal region of human APAF1. AA range:501-550

Specificity：Apaf-1 Polyclonal Antibody detects endogenous levels of Apaf-1 protein.

Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Source：Polyclonal, Rabbit,IgG

Dilution：WB 1:500 - 1:2000. IHC: 1:100-1:300. ELISA: 1:20000.. IF 1:50-200

Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration：1 mg/ml

Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)

Other Name：APAF1;KIAA0413;Apoptotic protease-activating factor 1;APAF-1

Observed Band(KD)：135kD

Background： This gene encodes a cytoplasmic protein that initiates apoptosis. This protein contains several copies of the WD-40 domain, a caspase recruitment domain (CARD), and an ATPase domain (NB-ARC). Upon binding cytochrome c and dATP, this protein forms an oligomeric apoptosome. The apoptosome binds and cleaves caspase 9 preproprotein, releasing its mature, activated form. Activated caspase 9 stimulates the subsequent caspase cascade that commits the cell to apoptosis. Alternative splicing results in several transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008],

Function：domain:The CARD domain mediates interaction with APIP.,function:Oligomeric Apaf-1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis. This activation requires ATP. Isoform 6 is less effective in inducing apoptosis.,induction:By E2F and p53 in apoptotic neurons.,similarity:Contains 1 CARD domain.,similarity:Contains 1 NB-ARC domain.,similarity:Contains 13 WD repeats.,subunit:Monomer. Oligomerizes upon binding of cytochrome c and dATP. Oligomeric Apaf-1 and pro-caspase-9 bind to each other via their respective NH2-terminal CARD domains and consecutively mature caspase-9 is released from the complex. Pro-caspase-3 is recruited into the Apaf-1-pro-caspase-9 complex via interaction with pro-caspase-9. Interacts with APIP.,tissue specificity:Ubiquitous. Highest levels of expression in adult spleen and per

Subcellular Location：Cytoplasm .

Expression：Ubiquitous. Highest levels of expression in adult spleen and peripheral blood leukocytes, and in fetal brain, kidney and lung. Isoform 1 is expressed in heart, kidney and liver.