Target：DNA pol β

Fields：Base excision repair;Human T-cell leukemia virus 1 infection;Viral carcinogenesis

Gene Name：POLB

Protein Name：DNA polymerase beta

Human Gene Id：5423

Human Swiss Prot No：P06746

Mouse Gene Id：18970

Mouse Swiss Prot No：Q8K409

Rat Gene Id：29240

Rat Swiss Prot No：P06766

Immunogen：The antiserum was produced against synthesized peptide derived from human DNA Polymerase beta. AA range:286-335

Specificity：DNA pol β Polyclonal Antibody detects endogenous levels of DNA pol β protein.

Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Source：Polyclonal, Rabbit,IgG

Dilution：WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:10000.. IF 1:50-200

Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration：1 mg/ml

Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)

Other Name：POLB;DNA polymerase beta

Observed Band(KD)：38kD

Background： The protein encoded by this gene is a DNA polymerase involved in base excision and repair, also called gap-filling DNA synthesis. The encoded protein, acting as a monomer, is normally found in the cytoplasm, but it translocates to the nucleus upon DNA damage. Several transcript variants of this gene exist, but the full-length nature of only one has been described to date. [provided by RefSeq, Sep 2011],

Function：catalytic activity:Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).,cofactor:Binds 2 magnesium ions per subunit.,domain:Residues 239-252 form a flexible loop which appears to affect the polymerase fidelity.,function:Repair polymerase. Conducts "gap-filling" DNA synthesis in a stepwise distributive fashion rather than in a processive fashion as for other DNA polymerases. Has a 5'-deoxyribose-5-phosphate lyase (dRP lyase) activity.,PTM:Methylation by PRMT6 stimulates the polymerase activity by enhancing DNA binding and processivity.,similarity:Belongs to the DNA polymerase type-X family.,subunit:Monomer.,

Subcellular Location：Nucleus. Cytoplasm. Cytoplasmic in normal conditions. Translocates to the nucleus following DNA damage.

Expression： Skin,Testis,