Target：DNA pol α

Fields：DNA replication

Gene Name：POLA1

Protein Name：DNA polymerase alpha catalytic subunit

Human Gene Id：5422

Human Swiss Prot No：P09884

Mouse Swiss Prot No：P33609

Immunogen：The antiserum was produced against synthesized peptide derived from human DNA Polymerase alpha. AA range:81-130

Specificity：DNA pol α Polyclonal Antibody detects endogenous levels of DNA pol α protein.

Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Source：Polyclonal, Rabbit,IgG

Dilution：WB 1:500 - 1:2000. ELISA: 1:20000. Not yet tested in other applications.

Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration：1 mg/ml

Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)

Other Name：POLA1;POLA;DNA polymerase alpha catalytic subunit;DNA polymerase alpha catalytic subunit p180

Observed Band(KD)：165kD

Background： This gene encodes the catalytic subunit of DNA polymerase, which together with a regulatory and two primase subunits, forms the DNA polymerase alpha complex. The catalytic subunit plays an essential role in the initiation of DNA replication. [provided by RefSeq, Mar 2010],

Function：catalytic activity:Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).,function:Polymerase alpha in a complex with DNA primase is a replicative polymerase.,miscellaneous:In eukaryotes there are five DNA polymerases: alpha, beta, gamma, delta, and epsilon which are responsible for different reactions of DNA synthesis.,PTM:A 165 kDa form is probably produced by proteolytic cleavage at Lys-124.,similarity:Belongs to the DNA polymerase type-B family.,subunit:Interacts with SV40 Large T antigen; this interaction allows viral DNA replication.,

Subcellular Location：Nucleus . Cytoplasm, cytosol . In the cytosol, colocalizes with RNA:DNA hybrids with a speckled pattern. .