Target：DBH

Fields：Tyrosine metabolism;Metabolic pathways

Gene Name：DBH

Protein Name：Dopamine beta-hydroxylase

Human Gene Id：1621

Human Swiss Prot No：P09172

Mouse Swiss Prot No：Q64237

Immunogen：The antiserum was produced against synthesized peptide derived from human DBH. AA range:401-450

Specificity：DBH Polyclonal Antibody detects endogenous levels of DBH protein.

Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Source：Polyclonal, Rabbit,IgG

Dilution：WB 1:500 - 1:2000. ELISA: 1:10000. Not yet tested in other applications.

Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration：1 mg/ml

Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)

Other Name：DBH;Dopamine beta-hydroxylase;Dopamine beta-monooxygenase

Observed Band(KD)：69kD

Background： The protein encoded by this gene is an oxidoreductase belonging to the copper type II, ascorbate-dependent monooxygenase family. It is present in the synaptic vesicles of postganglionic sympathetic neurons and converts dopamine to norepinephrine. It exists in both soluble and membrane-bound forms, depending on the absence or presence, respectively, of a signal peptide. [provided by RefSeq, Jul 2008],

Function：catalytic activity:3,4-dihydroxyphenethylamine + ascorbate + O(2) = noradrenaline + dehydroascorbate + H(2)O.,cofactor:Binds 1 PQQ per subunit.,cofactor:Binds 2 copper ions per subunit.,disease:Defects in DBH are the cause of DBH deficiency [MIM:223360]; also called norepinephrine deficiency or noradrenaline deficiency. This disorder is characterized by profound deficits in autonomic and cardiovascular function, but apparently only subtle signs, if any, of central nervous system dysfunction.,function:Conversion of dopamine to noradrenaline.,induction:Activity is enhanced by nerve growth factor (in superior cervical ganglia and adrenal medulla). Trans-synaptic stimulation with reserpine, acetylcholine and glucocorticoids.,online information:Dopamine beta hydroxylase entry,pathway:Catecholamine biosynthesis; norepinephrine biosynthesis; norepinephrine from dopamine: step 1/1.,polymorphism:

Subcellular Location：[Soluble dopamine beta-hydroxylase]: Cytoplasmic vesicle, secretory vesicle lumen . Cytoplasmic vesicle, secretory vesicle, chromaffin granule lumen . Secreted .; Cytoplasmic vesicle, secretory vesicle membrane ; Single-pass type II membrane protein . Cytoplasmic vesicle, secretory vesicle, chromaffin granule membrane ; Single-pass type II membrane protein .

Expression： Brain,Plasma,