Target：CRSP77

Fields：Thyroid hormone signaling pathway

Gene Name：MED17

Protein Name：Mediator of RNA polymerase II transcription subunit 17

Human Gene Id：9440

Human Swiss Prot No：Q9NVC6

Mouse Gene Id：234959

Mouse Swiss Prot No：Q8VCD5

Immunogen：The antiserum was produced against synthesized peptide derived from human MED17. AA range:141-190

Specificity：CRSP77 Polyclonal Antibody detects endogenous levels of CRSP77 protein.

Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Source：Polyclonal, Rabbit,IgG

Dilution：WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:10000.. IF 1:50-200

Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration：1 mg/ml

Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)

Other Name：MED17;ARC77;CRSP6;DRIP77;DRIP80;TRAP80;Mediator of RNA polymerase II transcription subunit 17;Activator-recruited cofactor 77 kDa component;ARC77;Cofactor required for Sp1 transcriptional activation subunit 6;CRSP complex subunit

Observed Band(KD)：73kD

Background： The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. [provided by RefSeq, Jul 2008],

Function：function:Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Belongs to the Mediator complex subunit 17 family.,subunit:Interacts with GATA1 and PPARG (By similarity). Component of the Mediator complex, which is composed of MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED2

Subcellular Location：Nucleus .

Expression：Ubiquitous.